Pseudomonas aeruginosa PAO1, PA5014 (glnE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5014
Name
glnE
Replicon chromosome
Genomic location 5632926 - 5635874 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253701.1
GI 15600207
Affymetrix PA5014_glnE_at
Entrez 881325
GenBank AAG08399.1
INSDC AAG08399.1
NCBI Locus Tag PA5014
protein_id(GenBank) gb|AAG08399.1|AE004913_14|gnl|PseudoCAP|PA5014
TIGR NTL03PA05015
UniParc UPI00000C5EC8
UniProtKB Acc Q9HUF4
UniProtKB ID GLNE_PSEAE
UniRef100 UniRef100_Q9HUF4
UniRef50 UniRef50_Q9HUF4
UniRef90 UniRef90_Q9HUF4

Product

Feature Type CDS
Coding Frame 1
Product Name
glutamate-ammonia-ligase adenylyltransferase
Product Name Confidence: Class 2
Synonyms Glutamine-synthetase adenylyltransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -22.63
Kyte-Doolittle Hydrophobicity Value -0.224
Molecular Weight (kDa) 110.1
Isoelectric Point (pI) 5.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 290 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004621 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5014
Search term: glnE

Human Homologs

References

Mutations that alter the covalent modification of glutamine synthetase in Salmonella typhimurium.
Bancroft S, Rhee SG, Neumann C, Kustu S
J Bacteriol 1978 Jun;134(3):1046-55
PubMed ID: 26663
The role of adenylyltransferase and uridylyltransferase in the regulation of glutamine synthetase in Escherichia coli.
Rhee SG, Park SC, Koo JH
Curr Top Cell Regul 1985;27:221-32
PubMed ID: 2868842
The genes of the glutamine synthetase adenylylation cascade are not regulated by nitrogen in Escherichia coli.
van Heeswijk WC, Rabenberg M, Westerhoff HV, Kahn D
Mol Microbiol 1993 Aug;9(3):443-57
PubMed ID: 8412694