Pseudomonas aeruginosa PAO1, PA5015 (aceE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5015
Name
aceE
Synonym: aceA
Replicon chromosome
Genomic location 5636156 - 5638804 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253702.1
GI 15600208
Affymetrix PA5015_aceE_at
Entrez 881326
GenBank AAG08400.1
INSDC AAG08400.1
NCBI Locus Tag PA5015
protein_id(GenBank) gb|AAG08400.1|AE004914_1|gnl|PseudoCAP|PA5015
TIGR NTL03PA05016
UniParc UPI0000130BF0
UniProtKB Acc Q59637
UniProtKB ID ODP1_PSEAE
UniRef100 UniRef100_Q59637
UniRef50 UniRef50_P10801
UniRef90 UniRef90_Q59637

Product

Feature Type CDS
Coding Frame 1
Product Name
pyruvate dehydrogenase
Product Name Confidence: Class 1
Synonyms pyruvate dehydrogenase complex component E1
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (99.56 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -17.07
Kyte-Doolittle Hydrophobicity Value -0.426
Molecular Weight (kDa) 99.6
Isoelectric Point (pI) 5.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 372 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004622 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5015
Search term: aceE

Human Homologs

References

Sequences and expression of pyruvate dehydrogenase genes from Pseudomonas aeruginosa.
Rae JL, Cutfield JF, Lamont IL
J Bacteriol 1997 Jun;179(11):3561-71
PubMed ID: 9171401
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344