Pseudomonas aeruginosa PAO1, PA5017 (dipA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5017
Name
dipA
Replicon chromosome
Genomic location 5641010 - 5643709 (+ strand)
Transposon Mutants 8 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentOverexpression of PA5017 impairs TTSS-mediated cytotoxicity

Cross-References

RefSeq NP_253704.1
GI 15600210
Affymetrix PA5017_at
Entrez 881298
GenBank AAG08402.1
INSDC AAG08402.1
NCBI Locus Tag PA5017
protein_id(GenBank) gb|AAG08402.1|AE004914_3|gnl|PseudoCAP|PA5017
TIGR NTL03PA05018
UniParc UPI00000C5EC9
UniProtKB Acc Q9HUF2
UniProtKB ID Q9HUF2_PSEAE
UniRef100 UniRef100_Q9HUF2
UniRef50 UniRef50_Q4KJD2
UniRef90 UniRef90_V8ER08

Product

Feature Type CDS
Coding Frame 1
Product Name
DipA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -16.26
Kyte-Doolittle Hydrophobicity Value -0.316
Molecular Weight (kDa) 101.3
Isoelectric Point (pI) 6.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Not available / Not known
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse model of acute pneumonia
Reference 24901523

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 493 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004624 (539 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5017
Search term: dipA
Search term: DipA

Human Homologs

References

The phosphodiesterase DipA (PA5017) is essential for Pseudomonas aeruginosa biofilm dispersion.
Roy AB, Petrova OE, Sauer K
J. Bacteriol. 2012 Jun;194(11):2904-15
PubMed ID: 22493016
BdlA, DipA and induced dispersion contribute to acute virulence and chronic persistence of Pseudomonas aeruginosa.
Li Y, Petrova OE, Su S, Lau GW, Panmanee W, Na R, Hassett DJ, Davies DG, Sauer K
PLoS Pathog. 2014 Jun;10(6):e1004168
PubMed ID: 24901523
Identification of a new gene PA5017 involved in flagella-mediated motility, chemotaxis and biofilm formation in Pseudomonas aeruginosa.
Li Y, Xia H, Bai F, Xu H, Yang L, Yao H, Zhang L, Zhang X, Bai Y, Saris PE, Tolker-Nielsen T, Qiao M
FEMS Microbiol Lett 2007 Jul;272(2):188-95
PubMed ID: 17521365