Pseudomonas aeruginosa PAO1, PA5117 (typA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5117
Name
typA
Synonym: bipA
Replicon chromosome
Genomic location 5762659 - 5764611 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentTranslation confirmed using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer. Coordinates verified using RT-PCR (PMID:24291602).

Cross-References

RefSeq NP_253804.1
GI 15600310
Affymetrix PA5117_typA_at
Entrez 879601
GenBank AAG08502.1
INSDC AAG08502.1
NCBI Locus Tag PA5117
protein_id(GenBank) gb|AAG08502.1|AE004924_7|gnl|PseudoCAP|PA5117
TIGR NTL03PA05118
UniParc UPI00000C5F0E
UniProtKB Acc Q9HU67
UniProtKB ID Q9HU67_PSEAE
UniRef100 UniRef100_Q9HU67
UniRef50 UniRef50_P0A3B2
UniRef90 UniRef90_Q9HU67

Product

Feature Type CDS
Coding Frame 1
Product Name
regulatory protein TypA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -16.36
Kyte-Doolittle Hydrophobicity Value -0.309
Molecular Weight (kDa) 66.9
Isoelectric Point (pI) 5.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Caenorhabditis elegans
Notes
Infection model C. elegans Infection model
Reference 23570569
Source 2
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Drosophila melanogaster
Notes
Infection model D. discoideum Infection model
Reference 23570569

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004710 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5117
Search term: typA

Human Homologs

Ensembl 110, assembly GRCh38.p14
GTP binding elongation factor GUF1 [Source:HGNC Symbol;Acc:HGNC:25799]
E-value: 3.0e-41
Percent Identity: 28.2

References

BipA: a tyrosine-phosphorylated GTPase that mediates interactions between enteropathogenic Escherichia coli (EPEC) and epithelial cells.
Farris M, Grant A, Richardson TB, O'Connor CD
Mol Microbiol 1998 Apr;28(2):265-79
PubMed ID: 9622352
Tyrosine phosphorylation in Escherichia coli.
Freestone P, Trinei M, Clarke SC, Nyström T, Norris V
J Mol Biol 1998 Jun 26;279(5):1045-51
PubMed ID: 9642082
Protein phosphorylation in Escherichia coli L. form NC-7.
Freestone P, Grant S, Trinei M, Onoda T, Norris V
Microbiology (Reading) 1998 Dec;144 ( Pt 12):3289-3295
PubMed ID: 9884220
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
TypA is involved in virulence, antimicrobial resistance and biofilm formation in Pseudomonas aeruginosa.
Neidig A, Yeung AT, Rosay T, Tettmann B, Strempel N, Rueger M, Lesouhaitier O, Overhage J
BMC Microbiol 2013 Apr 9;13:77
PubMed ID: 23570569
Identification of genes involved in swarming motility using a Pseudomonas aeruginosa PAO1 mini-Tn5-lux mutant library.
Overhage J, Lewenza S, Marr AK, Hancock RE
J Bacteriol 2007 Mar;189(5):2164-9
PubMed ID: 17158671
LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.
Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K
J Proteomics 2014 Jan 16;96:328-342
PubMed ID: 24291602