Pseudomonas aeruginosa PAO1, PA5203 (gshA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5203
Name
gshA
Replicon chromosome
Genomic location 5856532 - 5858115 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253890.1
GI 15600396
Affymetrix PA5203_gshA_at
Entrez 879474
GenBank AAG08588.1
INSDC AAG08588.1
NCBI Locus Tag PA5203
protein_id(GenBank) gb|AAG08588.1|AE004933_4|gnl|PseudoCAP|PA5203
TIGR NTL03PA05204
UniParc UPI000012BBB8
UniProtKB Acc Q9HTY6
UniProtKB ID GSH1_PSEAE
UniRef100 UniRef100_Q9HTY6
UniRef50 UniRef50_Q9HTY6
UniRef90 UniRef90_Q9HTY6

Product

Feature Type CDS
Coding Frame 1
Product Name
glutamate--cysteine ligase
Product Name Confidence: Class 2
Synonyms gamma-glutamylcysteine synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -16.05
Kyte-Doolittle Hydrophobicity Value -0.317
Molecular Weight (kDa) 59.2
Isoelectric Point (pI) 4.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 134 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004787 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5203
Search term: gshA

Human Homologs

References

The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli.
Watanabe K, Yamano Y, Murata K, Kimura A
Nucleic Acids Res 1986 Jun 11;14(11):4393-400
PubMed ID: 2872655
Functional analysis of the gamma-glutamylcysteine synthetase of Escherichia coli B: effect of substitution of His-150 to Ala.
Inoue Y, Iba Y, Yano H, Murata K, Kimura A
Appl. Microbiol. Biotechnol. 1993 Jan;38(4):473-7
PubMed ID: 7765288