Pseudomonas putida HB3267, B479_22560

Gene Feature Overview

Strain	Pseudomonas putida HB3267 GCF_000325725.1|latest
Locus Tag	B479_22560
Name
Replicon	chromosome
Genomic location	4963556 - 4964275 (- strand)

Cross-References

RefSeq	YP_007231344.1
GI	431804441
Entrez	14290892
INSDC	AGA75401.1
NCBI Locus Tag	B479_22560
UniParc	UPI00026D7445
UniProtKB Acc	L0FRM5
UniProtKB ID	L0FRM5_PSEPU
UniRef100	UniRef100_A0A099N6S0
UniRef50	UniRef50_P00437
UniRef90	UniRef90_P00437

Product

Feature Type	CDS
Coding Frame	1
Product Name	protocatechuate 3,4-dioxygenase subunit beta
Synonyms
Evidence for Translation
Charge (pH 7)	7.79
Kyte-Doolittle Hydrophobicity Value	-0.453
Molecular Weight (kDa)	26.8
Isoelectric Point (pI)	9.35

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3T67	OXIDOREDUCTASE	07/28/11	Axial Ligand Swapping In Double Mutant Maintains Intradiol-cleavage Chemistry in Protocatechuate 3,4-Dioxygenase	Pseudomonas putida	1.67	X-RAY DIFFRACTION	97.9
3PCJ	DIOXYGENASE	07/18/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 2-HYDROXYISONICOTINIC ACID N-OXIDE	Pseudomonas putida	2.13	X-RAY DIFFRACTION	97.9
3PCG	DIOXYGENASE	04/29/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH THE INHIBITOR 4-HYDROXYPHENYLACETATE	Pseudomonas putida	1.96	X-RAY DIFFRACTION	97.9
3PCC	DIOXYGENASE	04/29/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 4-HYDROXYBENZOATE	Pseudomonas putida	1.98	X-RAY DIFFRACTION	97.9
3PCI	DIOXYGENASE	07/02/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-IODO-4-HYDROXYBENZOATE	Pseudomonas putida	2.21	X-RAY DIFFRACTION	97.9
3PCF	DIOXYGENASE	06/27/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-FLURO-4-HYDROXYBENZOATE	Pseudomonas putida	2.15	X-RAY DIFFRACTION	97.9
3PCB	DIOXYGENASE	04/25/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-HYDROXYBENZOATE	Pseudomonas putida	2.19	X-RAY DIFFRACTION	97.9
3PCN	DIOXYGENASE	08/19/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3,4-DIHYDROXYPHENYLACETATE	Pseudomonas putida	2.4	X-RAY DIFFRACTION	97.9
3PCH	DIOXYGENASE	07/01/97	STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-CHLORO-4-HYDROXYBENZOATE	Pseudomonas putida	2.05	X-RAY DIFFRACTION	97.9

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
2-(3,4-Dihydroxyphenyl)Acetic Acid	DB01702	DrugBank	5.1.4	P00437	Protocatechuate 3,4-dioxygenase beta chain	97.9	239	2.2e-141

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 128 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000151 (503 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: B479_22560 Search term: protocatechuate 3,4-dioxygenase subunit beta

Human Homologs

References