Pseudomonas aeruginosa UCBPP-PA14, PA14_05540 (mexB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_05540
Name	mexB
Replicon	chromosome
Genomic location	487942 - 491082 (+ strand)
Transposon Mutants	2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	23 transposon mutants in orthologs

Cross-References

RefSeq	YP_788600.1
GI	116054157
Entrez	4384294
INSDC	ABJ15393.1
NCBI Locus Tag	PA14_05540
UniParc	UPI000012F022
UniProtKB Acc	Q02TY6
UniProtKB ID	Q02TY6_PSEAB
UniRef100	UniRef100_P52002
UniRef50	UniRef50_P52002
UniRef90	UniRef90_P52002

Product

Feature Type	CDS
Coding Frame	1
Product Name	RND multidrug efflux transporter MexB
Synonyms
Evidence for Translation
Charge (pH 7)	-9.89
Kyte-Doolittle Hydrophobicity Value	0.274
Molecular Weight (kDa)	112.8
Isoelectric Point (pI)	5.40

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name	Definition	Accession	Model Type (?)	SNP	Cutoff	Bit Score	Percent Identity	CARD Version
MexB	MexB is the inner membrane multidrug exporter of the efflux complex MexAB-OprM. [PMID:19361527, PMID:21178960, PMID:14973037, PMID:20583998]	ARO:3000378	protein homolog model		Strict	1984.5	99.9	3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6IIA	MEMBRANE PROTEIN	10/04/18	MexB in complex with LMNG	Pseudomonas aeruginosa PAO1	2.91	X-RAY DIFFRACTION	100.0
6IOL	MEMBRANE PROTEIN	10/30/18	Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state)	Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1	3.76	ELECTRON MICROSCOPY	100.0
2V50	MEMBRANE PROTEIN	10/01/08	The Missing Part of the Bacterial MexAB-OprM System: Structural determination of the Multidrug Exporter MexB	PSEUDOMONAS AERUGINOSA PA01	3	X-RAY DIFFRACTION	100.0
3W9I	MEMBRANE PROTEIN	04/04/13	Structural basis for the inhibition of bacterial multidrug exporters	Pseudomonas aeruginosa	2.71	X-RAY DIFFRACTION	100.0
6IOK	MEMBRANE PROTEIN	10/30/18	Cryo-EM structure of multidrug efflux pump MexAB-OprM (0 degree state)	Pseudomonas aeruginosa PAO1	3.64	ELECTRON MICROSCOPY	100.0
3W9J	MEMBRANE PROTEIN	04/04/13	Structural basis for the inhibition of bacterial multidrug exporters	Pseudomonas aeruginosa	3.15	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 488 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000417 (2093 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_05540 Search term: mexB Search term: RND multidrug efflux transporter MexB

Human Homologs

References