Pseudomonas aeruginosa UCBPP-PA14, PA14_07500 (rmcA)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
Locus Tag
Replicon chromosome
Genomic location 643347 - 647084 (- strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 9 transposon mutants in orthologs


RefSeq YP_788748.1
GI 116054303
Entrez 4384222
INSDC ABJ15539.1
NCBI Locus Tag PA14_07500
UniParc UPI000045D43F
UniProtKB Acc Q02TI8
UniRef100 UniRef100_Q02TI8
UniRef50 UniRef50_Q4K4W0
UniRef90 UniRef90_Q9I5W1


Feature Type CDS
Coding Frame 1
Product Name
redox regulator of c_di_GMP, RmcA
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -29.53
Kyte-Doolittle Hydrophobicity Value -0.176
Molecular Weight (kDa) 139.7
Isoelectric Point (pI) 5.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7EJG PEPTIDE BINDING PROTEIN 04/02/21 Crystal structure of PBP domain of RMCA Pseudomonas aeruginosa 1.68 X-RAY DIFFRACTION 98.7
5M3C HYDROLASE 10/14/16 Structure of the hybrid domain (GGDEF-EAL) of PA0575 from Pseudomonas aeruginosa PAO1 at 2.8 Ang. with GTP and Ca2+ bound to the active site of the GGDEF domain Pseudomonas aeruginosa 2.8 X-RAY DIFFRACTION 99.5

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 563 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000558 (491 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA14_07500
Search term: rmcA

Human Homologs


Insights into the GTP-dependent allosteric control of c-di-GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa.
Mantoni F, Paiardini A, Brunotti P, D'Angelo C, Cervoni L, Paone A, Cappellacci L, Petrelli R, Ricciutelli M, Leoni L, Rampioni G, Arcovito A, Rinaldo S, Cutruzzolà F, Giardina G
FEBS J 2018 Oct;285(20):3815-3834
PubMed ID: 30106221
Electron-shuttling antibiotics structure bacterial communities by modulating cellular levels of c-di-GMP.
Okegbe C, Fields BL, Cole SJ, Beierschmitt C, Morgan CJ, Price-Whelan A, Stewart RC, Lee VT, Dietrich LEP
Proc Natl Acad Sci U S A 2017 Jun 27;114(26):E5236-E5245
PubMed ID: 28607054