Pseudomonas aeruginosa M18, PAM18_0427 (oprM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Pseudomonas aeruginosa M18
GCF_000226155.1|latest
Locus Tag
PAM18_0427
Name
oprM
Replicon chromosome
Genomic location 469857 - 471314 (+ strand)

Cross-References

RefSeq YP_005973018.1
GI 386056496
Entrez 12564445
NCBI Locus Tag PAM18_0427

Product

Feature Type CDS
Coding Frame 1
Product Name
major intrinsic multiple antibiotic resistance efflux outer membrane protein OprM
Synonyms
Evidence for Translation
Charge (pH 7) -2.79
Kyte-Doolittle Hydrophobicity Value -0.215
Molecular Weight (kDa) 52.6
Isoelectric Point (pI) 5.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
OprM OprM is an outer membrane factor protein found in Pseudomonas aeruginosa and Burkholderia vietnamiensis. It is part of the MexAB-OprM, MexVW-OprM, MexXY-OprM and the AmrAB-OprM complex. [PMID:16508113, PMID:16511029, PMID:15797729] ARO:3000379 protein homolog model Perfect 916.8 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6TA6 ANTIMICROBIAL PROTEIN 10/29/19 MexAB assembly of the Pseudomonas MexAB-OprM efflux pump reconstituted in nanodiscs Pseudomonas aeruginosa 3.2 ELECTRON MICROSCOPY 100.0
6ZRE TRANSLOCASE 07/13/20 Deciphering the role of the channel constrictions in the opening mechanism of MexAB-OprM efflux pump from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.8 X-RAY DIFFRACTION 100.0
6IOL MEMBRANE PROTEIN 10/30/18 Cryo-EM structure of multidrug efflux pump MexAB-OprM (60 degree state) Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1 3.76 ELECTRON MICROSCOPY 100.0
4Y1K MEMBRANE PROTEIN 02/07/15 PALMITOYLATED OPRM OUTER MEMBRANE FACTOR Pseudomonas aeruginosa 3.8 X-RAY DIFFRACTION 100.0
1WP1 MEMBRANE PROTEIN 08/28/04 Crystal structure of the drug-discharge outer membrane protein, OprM Pseudomonas aeruginosa 2.56 X-RAY DIFFRACTION 100.0
7AKZ TRANSLOCASE 10/02/20 Deciphering the role of the channel constrictions in the opening mechanism of MexAB-OprM efflux pump from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.2 X-RAY DIFFRACTION 100.0
6IOK MEMBRANE PROTEIN 10/30/18 Cryo-EM structure of multidrug efflux pump MexAB-OprM (0 degree state) Pseudomonas aeruginosa PAO1 3.64 ELECTRON MICROSCOPY 100.0
6TA5 ANTIMICROBIAL PROTEIN 10/29/19 OprM-MexA complex from the MexAB-OprM Pseudomonas aeruginosa whole assembly reconstituted in nanodiscs Pseudomonas aeruginosa 3.2 ELECTRON MICROSCOPY 100.0
3D5K MEMBRANE PROTEIN 05/16/08 Crystal structure of the OprM channel in a non-symmetrical space group Pseudomonas aeruginosa 2.4 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 316 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000418 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PAM18_0427
Search term: oprM

Human Homologs

References

No references are associated with this feature.