Pseudomonas aeruginosa B136-33, G655_19595

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Pseudomonas aeruginosa B136-33
GCF_000359505.1|latest
Locus Tag
G655_19595
Name
Replicon chromosome
Genomic location 4318495 - 4318902 (- strand)

Cross-References

RefSeq YP_007710758.1
GI 478480107
Entrez 15136823
NCBI Locus Tag G655_19595

Product

Feature Type CDS
Coding Frame 1
Product Name
fosfomycin resistance protein
Synonyms
Evidence for Translation
Charge (pH 7) -0.87
Kyte-Doolittle Hydrophobicity Value -0.238
Molecular Weight (kDa) 15153.9
Isoelectric Point (pI) 6.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
FosA An enzyme that confers resistance to fosfomycin in Serratia marcescens by breaking the epoxide ring of the molecule. It depends on the cofactors Manganese (II) and Potassium and uses Glutathione (GSH) as the nucleophilic molecule. In Pseudomonas aeruginosa, FosA catalyzes the conjugation of glutathione to carbon-1 of fosfomycin, rendering it ineffective as an antibacterial drug. [PMID:15075406, PMID:15741169] ARO:3000149 protein homolog model Strict 282.3 99.26 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1LQK TRANSFERASE 05/10/02 High Resolution Structure of Fosfomycin Resistance Protein A (FosA) Pseudomonas aeruginosa 1.35 X-RAY DIFFRACTION 99.3
1NNR TRANSFERASE 01/14/03 Crystal structure of a probable fosfomycin resistance protein (PA1129) from Pseudomonas aeruginosa with sulfate present in the active site Pseudomonas aeruginosa 2.25 X-RAY DIFFRACTION 99.3
1LQO TRANSFERASE 05/11/02 Crystal Strutcure of the Fosfomycin Resistance Protein A (FosA) Containing Bound Thallium Cations Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 99.3
1NKI TRANSFERASE 01/03/03 CRYSTAL STRUCTURE OF THE FOSFOMYCIN RESISTANCE PROTEIN A (FOSA) CONTAINING BOUND PHOSPHONOFORMATE Pseudomonas aeruginosa 0.95 X-RAY DIFFRACTION 99.3
1LQP TRANSFERASE 05/11/02 CRYSTAL STRUCTURE OF THE FOSFOMYCIN RESISTANCE PROTEIN (FOSA) CONTAINING BOUND SUBSTRATE Pseudomonas aeruginosa 1.19 X-RAY DIFFRACTION 99.3

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 87 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001089 (344 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: G655_19595

Human Homologs

References

No references are associated with this feature.