Pseudomonas aeruginosa LESB58, PALES_00451 (exoT)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa LESB58
Locus Tag
Replicon chromosome
Genomic location 58592 - 59965 (+ strand)


RefSeq YP_002437656
GI 218888792
Entrez 7175181


Feature Type CDS
Coding Frame 1
Product Name
exoenzyme T
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -8.32
Kyte-Doolittle Hydrophobicity Value -0.319
Molecular Weight (kDa) 48530.1
Isoelectric Point (pI) 5.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Host Associated
Note: This is based on PSORTb V3.0 secondary localization prediction
Class 3
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4JMF TOXIN/CHAPERONE 03/14/13 Crystal structure of ExoT (residues 28 -77)- SpcS complex from Pseudomonas aeruginosa at 2.1 angstrom Pseudomonas aeruginosa 2.099 X-RAY DIFFRACTION 100.0
6JNP TOXIN 03/17/19 Structure of ExoT-SpcS Complex from Pseudomonas aeruginosa in 2.2 Angstrom Pseudomonas aeruginosa 2.261 X-RAY DIFFRACTION 98.2
6GNN TOXIN 05/31/18 Exoenzyme T from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, tetrameric crystal form bound to STO1101 Homo sapiens; Pseudomonas aeruginosa PAO1 3.79 X-RAY DIFFRACTION 99.6

Virulence Evidence

Source 1
Database Victors : 4446
Category T3SS translocated effectors
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Cricetulus griseus
Notes exoT interferes with Rho signal transduction pathways, which regulate actin organization, exocytosis, cell cycle progression, and phagocytosis
Reference 10992524

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 8 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000044 (294 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PALES_00451
Search term: exoT
Search term: exoenzyme T

Human Homologs


No references are associated with this feature.