Pseudomonas aeruginosa LESB58, PALES_00451 (exoT)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa LESB58
GCF_000026645.1|latest |
| Locus Tag |
PALES_00451
|
| Name |
exoT
|
| Replicon | chromosome |
| Genomic location | 58592 - 59965 (+ strand) |
Cross-References
| RefSeq | YP_002437656 |
| GI | 218888792 |
| Entrez | 7175181 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
exoenzyme T
|
| Synonyms | |
| Evidence for Translation | |
| Charge (pH 7) | -8.32 |
| Kyte-Doolittle Hydrophobicity Value | -0.319 |
| Molecular Weight (kDa) | 48530.1 |
| Isoelectric Point (pI) | 5.66 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold 2 Protein Structure Predictions
Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4JMF | TOXIN/CHAPERONE | 03/14/13 | Crystal structure of ExoT (residues 28 -77)- SpcS complex from Pseudomonas aeruginosa at 2.1 angstrom | Pseudomonas aeruginosa | 2.099 | X-RAY DIFFRACTION | 100.0 |
| 6JNP | TOXIN | 03/17/19 | Structure of ExoT-SpcS Complex from Pseudomonas aeruginosa in 2.2 Angstrom | Pseudomonas aeruginosa | 2.261 | X-RAY DIFFRACTION | 98.2 |
| 6GNN | TOXIN | 05/31/18 | Exoenzyme T from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, tetrameric crystal form bound to STO1101 | Homo sapiens; Pseudomonas aeruginosa PAO1 | 3.79 | X-RAY DIFFRACTION | 99.6 |
Virulence Evidence
| Source 1 | |
| Database | Victors : 4446 |
| Category | T3SS translocated effectors |
| Evidence |
ECO:0000314
direct assay evidence used in manual assertion
|
| Host Organism | Cricetulus griseus |
| Notes | exoT interferes with Rho signal transduction pathways, which regulate actin organization, exocytosis, cell cycle progression, and phagocytosis |
| Reference | 10992524 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 8 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000044 (294 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PALES_00451
Search term: exoT
Search term: exoenzyme T
|
Human Homologs
References
No references are associated with this feature.