Pseudomonas aeruginosa PAO1, PA0011 (htrB1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0011
Name
htrB1
Synonym: 2-OH-lauroyltransferase
Replicon chromosome
Genomic location 14235 - 15122 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248701.1
GI 15595209
Affymetrix PA0011_at
Entrez 877921
GenBank AAG03401.1
INSDC AAG03401.1
NCBI Locus Tag PA0011
protein_id(GenBank) gb|AAG03401.1|AE004441_2|gnl|PseudoCAP|PA0011
TIGR NTL03PA00012
UniParc UPI00000C4EE6
UniProtKB Acc Q9I7B5
UniProtKB ID Q9I7B5_PSEAE
UniRef100 UniRef100_W0YKN8
UniRef50 UniRef50_C3KE43
UniRef90 UniRef90_W0YKN8

Product

Feature Type CDS
Coding Frame 1
Product Name
acyltransferase HtrB1
Product Name Confidence: Class 1
Synonyms HtrB1
2-OH-lauroyltransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 11.60
Kyte-Doolittle Hydrophobicity Value -0.094
Molecular Weight (kDa) 33.0
Isoelectric Point (pI) 9.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 281 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000011 (538 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0011
Search term: htrB1

Human Homologs

References

Biosynthesis of lipid A in Escherichia coli. Acyl carrier protein-dependent incorporation of laurate and myristate.
Brozek KA, Raetz CR
J Biol Chem 1990 Sep 15;265(26):15410-7
PubMed ID: 2203778
Structural characterization of the lipid A component of Pseudomonas aeruginosa wild-type and rough mutant lipopolysaccharides.
Kulshin VA, Zähringer U, Lindner B, Jäger KE, Dmitriev BA, Rietschel ET
Eur J Biochem 1991 Jun 15;198(3):697-704
PubMed ID: 1904818
Endotoxin biosynthesis in Pseudomonas aeruginosa: enzymatic incorporation of laurate before 3-deoxy-D-manno-octulosonate.
Mohan S, Raetz CR
J Bacteriol 1994 Nov;176(22):6944-51
PubMed ID: 7961456
Characterization of a novel gene related to antibiotic susceptibility in Pseudomonas aeruginosa.
Shen L, Ma Y, Liang H
J Antibiot (Tokyo) 2012 Feb;65(2):59-65
PubMed ID: 22146126
Function of the htrB high temperature requirement gene of Escherichia coli in the acylation of lipid A: HtrB catalyzed incorporation of laurate.
Clementz T, Bednarski JJ, Raetz CR
J Biol Chem 1996 May 17;271(20):12095-102
PubMed ID: 8662613