Pseudomonas aeruginosa PAO1, PA0350 (folA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0350
Name
folA
Synonym: tmrA
Replicon chromosome
Genomic location 393308 - 393814 (+ strand)

Cross-References

RefSeq NP_249041.1
GI 15595547
Affymetrix PA0350_folA_at
Entrez 879233
GenBank AAG03739.1
INSDC AAG03739.1
NCBI Locus Tag PA0350
protein_id(GenBank) gb|AAG03739.1|AE004473_1|gnl|PseudoCAP|PA0350
TIGR NTL03PA00351
UniParc UPI00000C501E
UniProtKB Acc Q9I6E3
UniProtKB ID Q9I6E3_PSEAE
UniRef100 UniRef100_W0YMU2
UniRef50 UniRef50_Q70YQ6
UniRef90 UniRef90_B7V2R3

Product

Feature Type CDS
Coding Frame 1
Product Name
dihydrofolate reductase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.52
Kyte-Doolittle Hydrophobicity Value 0.021
Molecular Weight (kDa) 18.2
Isoelectric Point (pI) 5.30

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 409 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000342 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0350
Search term: folA

Human Homologs

References

Purification and properties of Escherichia coli dihydrofolate reductase.
Baccanari D, Phillips A, Smith S, Sinski D, Burchall J
Biochemistry 1975 Dec 2;14(24):5267-73
PubMed ID: 46
Crystal structure of unliganded Escherichia coli dihydrofolate reductase. Ligand-induced conformational changes and cooperativity in binding.
Bystroff C, Kraut J
Biochemistry 1991 Feb 26;30(8):2227-39
PubMed ID: 1998681