Pseudomonas aeruginosa PAO1, PA0353 (ilvD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0353
Name
ilvD
Replicon chromosome
Genomic location 396057 - 397895 (- strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249044.1
GI 15595550
Affymetrix PA0353_ilvD_at
Entrez 879274
GenBank AAG03742.1
INSDC AAG03742.1
NCBI Locus Tag PA0353
protein_id(GenBank) gb|AAG03742.1|AE004473_4|gnl|PseudoCAP|PA0353
TIGR NTL03PA00354
UniParc UPI000012D585
UniProtKB Acc Q9I6E0
UniProtKB ID ILVD_PSEAE
UniRef100 UniRef100_B7V2R6
UniRef50 UniRef50_Q8XAV1
UniRef90 UniRef90_Q87V83

Product

Feature Type CDS
Coding Frame 1
Product Name
dihydroxy-acid dehydratase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -9.52
Kyte-Doolittle Hydrophobicity Value -0.110
Molecular Weight (kDa) 65.2
Isoelectric Point (pI) 5.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 574 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000344 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0353
Search term: ilvD

Human Homologs

References

The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12.
Cox JL, Cox BJ, Fidanza V, Calhoun DH
Gene 1987;56(2-3):185-98
PubMed ID: 3315862
The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase.
Flint DH, Emptage MH, Finnegan MG, Fu W, Johnson MK
J Biol Chem 1993 Jul 15;268(20):14732-42
PubMed ID: 8325851
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994