Pseudomonas aeruginosa PAO1, PA0482 (glcB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA0482
Name	glcB Synonym: aceB
Replicon	chromosome
Genomic location	542170 - 544347 (- strand)
Transposon Mutants	8 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_249173.1
GI	15595679
Affymetrix	PA0482_glcB_at
Entrez	879477
GenBank	AAG03871.1
INSDC	AAG03871.1
NCBI Locus Tag	PA0482
protein_id(GenBank)	gb|AAG03871.1|AE004485_12|gnl|PseudoCAP|PA0482
TIGR	NTL03PA00483
UniParc	UPI00000C507F
UniProtKB Acc	Q9I636
UniProtKB ID	MASZ_PSEAE
UniRef100	UniRef100_Q9I636
UniRef50	UniRef50_P37330
UniRef90	UniRef90_Q9I636

Product

Feature Type	CDS
Coding Frame	1
Product Name	malate synthase G Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	-14.19
Kyte-Doolittle Hydrophobicity Value	-0.251
Molecular Weight (kDa)	78.7
Isoelectric Point (pI)	5.45

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
5VFB	TRANSFERASE	04/07/17	1.36 Angstrom Resolution Crystal Structure of Malate Synthase G from Pseudomonas aeruginosa in Complex with Glycolic Acid.	Pseudomonas aeruginosa (strain PAO1)	1.36	X-RAY DIFFRACTION	100.0
5OAS	TRANSFERASE	06/23/17	Crystal structure of malate synthase G from Pseudomonas aeruginosa in apo form.	Pseudomonas aeruginosa	1.62	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 142 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000471 (534 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA0482 Search term: glcB Search term: malate synthase G

Human Homologs

References

Molecular characterization of Escherichia coli malate synthase G. Differentiation with the malate synthase A isoenzyme. Molina I, Pellicer MT, Badia J, Aguilar J, Baldoma L Eur J Biochem 1994 Sep 1;224(2):541-8 PubMed ID: 7925370

Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme. Reinscheid DJ, Eikmanns BJ, Sahm H Microbiology (Reading) 1994 Nov;140 ( Pt 11):3099-108 PubMed ID: 7812449

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Biochemical characterization of malate synthase G of P. aeruginosa. Roucourt B, Minnebo N, Augustijns P, Hertveldt K, Volckaert G, Lavigne R BMC Biochem 2009 Jun 24;10:20 PubMed ID: 19549344