Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0785
|
Name |
azoR1
Synonym: azoR |
Replicon | chromosome |
Genomic location | 857998 - 858636 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
RefSeq | NP_249476.1 |
GI | 15595982 |
Affymetrix | PA0785_at |
DNASU | PaCD00006357 |
Entrez | 882036 |
GenBank | AAG04174.1 |
INSDC | AAG04174.1 |
NCBI Locus Tag | PA0785 |
protein_id(GenBank) | gb|AAG04174.1|AE004513_6|gnl|PseudoCAP|PA0785 |
TIGR | NTL03PA00786 |
UniParc | UPI00001251D2 |
UniProtKB Acc | Q9I5F3 |
UniProtKB ID | AZOR1_PSEAE |
UniRef100 | UniRef100_Q9I5F3 |
UniRef50 | UniRef50_Q4KJ26 |
UniRef90 | UniRef90_Q9I5F3 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
FMN-dependent NADH-azoreductase 1, AzoR1
|
Synonyms |
azobenzene reductase, AzoR |
Evidence for Translation | |
Charge (pH 7) | -2.83 |
Kyte-Doolittle Hydrophobicity Value | -0.100 |
Molecular Weight (kDa) | 23.0 |
Isoelectric Point (pI) | 6.02 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3KEG | OXIDOREDUCTASE | 10/26/09 | X-ray Crystallographic Structure of a Y131F mutant of Pseudomonas Aeruginosa Azoreductase in complex with Methyl RED | Pseudomonas aeruginosa | 2.1 | X-RAY DIFFRACTION | 99.5 |
2V9C | OXIDOREDUCTASE | 08/23/07 | X-ray Crystallographic Structure of a Pseudomonas aeruginosa Azoreductase in Complex with Methyl Red. | PSEUDOMONAS AERUGINOSA | 2.18 | X-RAY DIFFRACTION | 100.0 |
3R6W | OXIDOREDUCTASE | 03/22/11 | paAzoR1 binding to nitrofurazone | Pseudomonas aeruginosa | 2.085 | X-RAY DIFFRACTION | 100.0 |
4N65 | OXIDOREDUCTASE | 10/11/13 | Crystal structure of paAzoR1 bound to anthraquinone-2-sulphonate | Pseudomonas aeruginosa | 1.816 | X-RAY DIFFRACTION | 100.0 |
3LT5 | OXIDOREDUCTASE | 02/15/10 | X-ray Crystallographic structure of a Pseudomonas Aeruginosa Azoreductase in complex with balsalazide | Pseudomonas aeruginosa | 2.3 | X-RAY DIFFRACTION | 100.0 |
4N9Q | OXIDOREDUCTASE | 10/21/13 | Crystal structure of paAzoR1 bound to ubiquinone-1 | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
2-(4-DIMETHYLAMINOPHENYL)DIAZENYLBENZOIC ACID | DB08209 | DrugBank | 5.1.4 | Q9I5F3 | FMN-dependent NADH-azoreductase 1 | 100.0 | 212 | 5.8e-114 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 245 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000759 (393 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0785
Search term: azoR1
Search term: FMN-dependent NADH-azoreductase 1, AzoR1
|
Isolation and properties of acyl carrier protein phosphodiesterase of Escherichia coli.
Fischl AS, Kennedy EP
J Bacteriol 1990 Sep;172(9):5445-9
PubMed ID: 2168383
|
Molecular cloning, characterisation and ligand-bound structure of an azoreductase from Pseudomonas aeruginosa.
Wang CJ, Hagemeier C, Rahman N, Lowe E, Noble M, Coughtrie M, Sim E, Westwood I
J Mol Biol 2007 Nov 9;373(5):1213-28
PubMed ID: 17904577
|
A novel mechanism for azoreduction.
Ryan A, Laurieri N, Westwood I, Wang CJ, Lowe E, Sim E
J Mol Biol 2010 Jul 2;400(1):24-37
PubMed ID: 20417637
|