Pseudomonas aeruginosa PAO1, PA0854 (fumC2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0854
Name
fumC2
Replicon chromosome
Genomic location 932718 - 934112 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249545.1
GI 15596051
Affymetrix PA0854_fumC2_at
Entrez 878044
GenBank AAG04243.1
INSDC AAG04243.1
NCBI Locus Tag PA0854
protein_id(GenBank) gb|AAG04243.1|AE004520_6|gnl|PseudoCAP|PA0854
TIGR NTL03PA00855
UniParc UPI00000C518D
UniProtKB Acc Q9I587
UniProtKB ID FUMC2_PSEAE
UniRef100 UniRef100_Q9I587
UniRef50 UniRef50_P05042
UniRef90 UniRef90_Q9I587

Product

Feature Type CDS
Coding Frame 1
Product Name
fumarate hydratase
Product Name Confidence: Class 2
Synonyms fumarase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.26
Kyte-Doolittle Hydrophobicity Value -0.035
Molecular Weight (kDa) 49.1
Isoelectric Point (pI) 6.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 576 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000821 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0854
Search term: fumC2

Human Homologs

Ensembl 110, assembly GRCh38.p14
fumarate hydratase [Source:HGNC Symbol;Acc:HGNC:3700]
E-value: 5.2e-152
Percent Identity: 59.2

References

Two biochemically distinct classes of fumarase in Escherichia coli.
Woods SA, Schwartzbach SD, Guest JR
Biochim Biophys Acta 1988 Apr 28;954(1):14-26
PubMed ID: 3282546
Crystallographic studies of the catalytic and a second site in fumarase C from Escherichia coli.
Weaver T, Banaszak L
Biochemistry 1996 Nov 5;35(44):13955-65
PubMed ID: 8909293