Pseudomonas aeruginosa PAO1, PA0869 (pbpG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0869
Name
pbpG
Replicon chromosome
Genomic location 949716 - 950648 (- strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249560.1
GI 15596066
Affymetrix PA0869_pbpG_at
Entrez 877694
GenBank AAG04258.1
INSDC AAG04258.1
NCBI Locus Tag PA0869
protein_id(GenBank) gb|AAG04258.1|AE004521_9|gnl|PseudoCAP|PA0869
TIGR NTL03PA00870
UniParc UPI0000131395
UniProtKB Acc P72161
UniProtKB ID PBP5_PSEAE
UniRef100 UniRef100_P72161
UniRef50 UniRef50_P72161
UniRef90 UniRef90_P72161

Product

Feature Type CDS
Coding Frame 1
Product Name
D-alanyl-D-alanine-endopeptidase
Product Name Confidence: Class 1
Synonyms penicillin-binding protein 7
D-ala-D-ala-carboxypeptidase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 20.40
Kyte-Doolittle Hydrophobicity Value -0.109
Molecular Weight (kDa) 34.0
Isoelectric Point (pI) 11.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 2
Additional evidence for subcellular localization

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Ceftazidime DB00438 DrugBank 5.1.4 P72161 D-alanyl-D-alanine endopeptidase 100.0 310 1.8e-167

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 376 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000836 (550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0869
Search term: pbpG

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Penicillin-binding protein 7/8 of Escherichia coli is a DD-endopeptidase.
Romeis T, Höltje JV
Eur. J. Biochem. 1994 Sep;224(2):597-604
PubMed ID: 7925376
Comparative analysis of Pseudomonas aeruginosa penicillin-binding protein 7 in the context of its membership in the family of low-molecular-mass PBPs.
Song J, Xie G, Elf PK, Young KD, Jensen RA
Microbiology (Reading, Engl.) 1998 Apr;144 ( Pt 4)
PubMed ID: 9579071