Pseudomonas aeruginosa PAO1, PA0934 (relA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 1023053 - 1025296 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs


RefSeq NP_249625.1
GI 15596131
Affymetrix PA0934_relA_at
Entrez 881299
GenBank AAG04323.1
INSDC AAG04323.1
NCBI Locus Tag PA0934
protein_id(GenBank) gb|AAG04323.1|AE004528_1|gnl|PseudoCAP|PA0934
UniParc UPI00000C51C6
UniProtKB Acc Q9I524
UniRef100 UniRef100_Q9I524
UniRef50 UniRef50_D7I2K8
UniRef90 UniRef90_A0A024HCY6


Feature Type CDS
Coding Frame 1
Product Name
GTP pyrophosphokinase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.82
Kyte-Doolittle Hydrophobicity Value -0.312
Molecular Weight (kDa) 83.8
Isoelectric Point (pI) 7.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Stringent Response
Evidence ECO:0000316
genetic interaction evidence used in manual assertion
Host Organism Rattus norvegicus
Notes spoT relA double mutant was significantly less virulent than both wild-type strain PAO1 and the relA mutant in D. melanogaster feeding assay.
Infection model Drosophila melanogaster feeding assay
Reference 21788391
Source 2
Database Victors : 4255
Category Stringent Response
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Drosophila melanogaster
Notes relA mutant attenuated for virulence in D. melanogaster feeding assay
Infection model Drosophila melanogaster feeding assay
Reference 15385461

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 565 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000900 (536 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0934
Search term: relA

Human Homologs


The nucleotide sequence and characterization of the relA gene of Escherichia coli.
Metzger S, Dror IB, Aizenman E, Schreiber G, Toone M, Friesen JD, Cashel M, Glaser G
J Biol Chem 1988 Oct 25;263(30):15699-704
PubMed ID: 2844820
Pseudomonas aeruginosa relA contributes to virulence in Drosophila melanogaster.
Erickson DL, Lines JL, Pesci EC, Venturi V, Storey DG
Infect Immun 2004 Oct;72(10):5638-45
PubMed ID: 15385461
The stringent response is essential for Pseudomonas aeruginosa virulence in the rat lung agar bead and Drosophila melanogaster feeding models of infection.
Vogt SL, Green C, Stevens KM, Day B, Erickson DL, Woods DE, Storey DG
Infect Immun 2011 Oct;79(10):4094-104
PubMed ID: 21788391