Pseudomonas aeruginosa PAO1, PA0945 (purM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0945
Name
purM
Replicon chromosome
Genomic location 1032763 - 1033824 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249636.1
GI 15596142
Affymetrix PA0945_purM_at
DNASU PaCD00006433
Entrez 879549
GenBank AAG04334.1
INSDC AAG04334.1
NCBI Locus Tag PA0945
protein_id(GenBank) gb|AAG04334.1|AE004528_12|gnl|PseudoCAP|PA0945
TIGR NTL03PA00946
UniParc UPI00000C51D1
UniProtKB Acc Q9I513
UniProtKB ID PUR5_PSEAE
UniRef100 UniRef100_Q9I513
UniRef50 UniRef50_Q8XAC5
UniRef90 UniRef90_Q9I513

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphoribosylaminoimidazole synthetase
Product Name Confidence: Class 2
Synonyms phosphoribosylformylglycinamidine cyclo-ligase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.06
Kyte-Doolittle Hydrophobicity Value 0.120
Molecular Weight (kDa) 37.1
Isoelectric Point (pI) 4.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 606 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000911 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0945
Search term: purM

Human Homologs

Ensembl 110, assembly GRCh38.p14
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase [Source:HGNC Symbol;Acc:HGNC:4163]
E-value: 1.1e-93
Percent Identity: 50.1
Ensembl 110, assembly GRCh38.p14
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase [Source:HGNC Symbol;Acc:HGNC:4163]
E-value: 1.1e-93
Percent Identity: 50.1
Ensembl 110, assembly GRCh38.p14
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase [Source:HGNC Symbol;Acc:HGNC:4163]
E-value: 1.1e-93
Percent Identity: 50.1

References

Nucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12.
Smith JM, Daum HA 3rd
J Biol Chem 1986 Aug 15;261(23):10632-6
PubMed ID: 3015935
Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12.
Smith JM, Daum HA 3rd
J Biol Chem 1987 Aug 5;262(22):10565-9
PubMed ID: 3301838