Pseudomonas aeruginosa PAO1, PA0963 (aspS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0963
Name
aspS
Synonym: syd
Replicon chromosome
Genomic location 1048459 - 1050234 (+ strand)

Cross-References

RefSeq NP_249654.1
GI 15596160
Affymetrix PA0963_aspS_at
Entrez 881917
GenBank AAG04352.1
INSDC BAA11815.1
NCBI Locus Tag PA0963
protein_id(GenBank) gb|AAG04352.1|AE004530_5|gnl|PseudoCAP|PA0963
TIGR NTL03PA00964
UniParc UPI00001363AB
UniProtKB Acc Q51422
UniProtKB ID SYDND_PSEAE
UniRef100 UniRef100_B7UXW9
UniRef50 UniRef50_Q83BE5
UniRef90 UniRef90_C1DRG2

Product

Feature Type CDS
Coding Frame 1
Product Name
aspartyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms aspRS
aspartate-tRNA ligase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (66.21 kDa, PMID:21751344)
Charge (pH 7) -13.46
Kyte-Doolittle Hydrophobicity Value -0.297
Molecular Weight (kDa) 66.2
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4WJ4 LIGASE/RNA 09/29/14 Crystal structure of non-discriminating aspartyl-tRNA synthetase from Pseudomonas aeruginosa complexed with tRNA(Asn) and aspartic acid Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 3.294 X-RAY DIFFRACTION 100.0
4WJ3 LIGASE/RNA 09/29/14 Crystal structure of the asparagine transamidosome from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 3.705 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000928 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0963
Search term: aspS

Human Homologs

Ensembl 110, assembly GRCh38.p14
aspartyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:25538]
E-value: 2.8e-118
Percent Identity: 39.9

References

Aspartyl-tRNA synthetase from Escherichia coli: cloning and characterisation of the gene, homologies of its translated amino acid sequence with asparaginyl- and lysyl-tRNA synthetases.
Eriani G, Dirheimer G, Gangloff J
Nucleic Acids Res 1990 Dec 11;18(23):7109-18
PubMed ID: 2129559
Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease.
Takahagi M, Iwasaki H, Nakata A, Shinagawa H
J Bacteriol 1991 Sep;173(18):5747-53
PubMed ID: 1885548
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344