Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1010
|
Name |
dapA
|
Replicon | chromosome |
Genomic location | 1093251 - 1094129 (+ strand) |
RefSeq | NP_249701.1 |
GI | 15596207 |
Affymetrix | PA1010_dapA_at |
Entrez | 879525 |
GenBank | AAG04399.1 |
INSDC | AAG04399.1 |
NCBI Locus Tag | PA1010 |
protein_id(GenBank) | gb|AAG04399.1|AE004533_10|gnl|PseudoCAP|PA1010 |
TIGR | NTL03PA01011 |
UniParc | UPI0000128E58 |
UniProtKB Acc | Q9I4W3 |
UniProtKB ID | DAPA_PSEAE |
UniRef100 | UniRef100_Q9I4W3 |
UniRef50 | UniRef50_Q9JZR4 |
UniRef90 | UniRef90_Q9I4W3 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
dihydrodipicolinate synthase
|
Synonyms | |
Evidence for Translation |
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -2.44 |
Kyte-Doolittle Hydrophobicity Value | 0.043 |
Molecular Weight (kDa) | 31.4 |
Isoelectric Point (pI) | 6.40 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3QZE | LYASE | 03/05/11 | Crystal Structure of DapA (PA1010) at 1.6 A resolution | Pseudomonas aeruginosa | 1.59 | X-RAY DIFFRACTION | 100.0 |
3PS7 | LYASE | 12/01/10 | Biochemical studies and crystal structure determination of dihydrodipicolinate synthase from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2.85 | X-RAY DIFFRACTION | 98.6 |
6P90 | STRUCTURAL PROTEIN | 06/08/19 | Crystal structure of PaDHDPS2-H56Q mutant | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 99.7 |
3PUO | LYASE | 12/06/10 | Crystal structure of dihydrodipicolinate synthase from Pseudomonas aeruginosa(PsDHDPS)complexed with L-lysine at 2.65A resolution | Pseudomonas aeruginosa | 2.65 | X-RAY DIFFRACTION | 98.6 |
3S8H | LYASE | 05/28/11 | Structure of dihydrodipicolinate synthase complexed with 3-Hydroxypropanoic acid(HPA)at 2.70 A resolution | Pseudomonas aeruginosa | 2.7 | X-RAY DIFFRACTION | 98.6 |
3NOE | LYASE | 06/25/10 | Crystal Structure of Dihydrodipicolinate synthase from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2.95 | X-RAY DIFFRACTION | 98.6 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 633 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000973 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1010
Search term: dapA
Search term: dihydrodipicolinate synthase
|
Reaction mechanism of Escherichia coli dihydrodipicolinate synthase investigated by X-ray crystallography and NMR spectroscopy.
Blickling S, Renner C, Laber B, Pohlenz HD, Holak TA, Huber R
Biochemistry 1997 Jan 7;36(1):24-33
PubMed ID: 8993314
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|