Pseudomonas aeruginosa PAO1, PA1046

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1046
Name
Replicon chromosome
Genomic location 1131674 - 1133947 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Comment 99% identical to beta-1,3-glucanase (GenBank: KY865343.1) in P. aeruginosa CAU 342A
CommentStructural similarity to 4BQ3: B-agarase (root-mean-square deviation: 0.8 angstroms)

Cross-References

RefSeq NP_249737.1
GI 15596243
Affymetrix PA1046_at
Entrez 879650
GenBank AAG04435.1
INSDC AAG04435.1
NCBI Locus Tag PA1046
protein_id(GenBank) gb|AAG04435.1|AE004536_14|gnl|PseudoCAP|PA1046
TIGR NTL03PA01047
UniParc UPI00000C5220
UniProtKB Acc Q9I4S8
UniProtKB ID Q9I4S8_PSEAE
UniRef100 UniRef100_Q9I4S8
UniRef50 UniRef50_B1J1C1
UniRef90 UniRef90_W0YPS0

Product

Feature Type CDS
Coding Frame 1
Product Name
beta-agarase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -5.37
Kyte-Doolittle Hydrophobicity Value -0.204
Molecular Weight (kDa) 82.7
Isoelectric Point (pI) 6.18

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 31 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001008 (450 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1046
Search term: beta-agarase

Human Homologs

References

Cloning and sequencing of agaA, a unique agarase 0107 gene from a marine bacterium, Vibrio sp. strain JT0107.
Sugano Y, Matsumoto T, Kodama H, Noma M
Appl Environ Microbiol 1993 Nov;59(11):3750-6
PubMed ID: 8285681
Sequence analysis of the agaB gene encoding a new beta-agarase from Vibrio sp. strain JT0107.
Sugano Y, Matsumoto T, Noma M
Biochim Biophys Acta 1994 May 17;1218(1):105-8
PubMed ID: 8193156
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799