Pseudomonas aeruginosa PAO1, PA1156 (nrdA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 1251418 - 1254309 (- strand)


RefSeq NP_249847.1
GI 15596353
Affymetrix PA1156_nrdA_at
Entrez 877716
GenBank AAG04545.1
INSDC AAG04545.1
NCBI Locus Tag PA1156
protein_id(GenBank) gb|AAG04545.1|AE004545_8|gnl|PseudoCAP|PA1156
UniParc UPI00000C527A
UniProtKB Acc Q9I4I1
UniRef100 UniRef100_W0YQ69
UniRef50 UniRef50_Q607W9
UniRef90 UniRef90_A4VK21


Feature Type CDS
Coding Frame 1
Product Name
NrdA, catalytic component of class Ia ribonucleotide reductase
Product Name Confidence: Class 1
Synonyms NrdA, catalytic component of class Ia ribonucleotide reductase
Evidence for Translation
Detected using LC-MS/MS (107.11 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -14.68
Kyte-Doolittle Hydrophobicity Value -0.309
Molecular Weight (kDa) 107.1
Isoelectric Point (pI) 5.69

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5IM3 OXIDOREDUCTASE 03/05/16 Crystal structure of the class I ribonucleotide reductase from Pseudomonas aeruginosa in complex with dATP Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.298 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 603 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001115 (534 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1156
Search term: nrdA

Human Homologs

Ensembl 110, assembly GRCh38.p14
ribonucleotide reductase catalytic subunit M1 [Source:HGNC Symbol;Acc:HGNC:10451]
E-value: 2.9e-157
Percent Identity: 41.0


Ribonucleotide reductase modularity: Atypical duplication of the ATP-cone domain in Pseudomonas aeruginosa.
Torrents E, Westman M, Sahlin M, Sjöberg BM
J Biol Chem 2006 Sep 1;281(35):25287-96
PubMed ID: 16829681
Promoter identification and expression analysis of Salmonella typhimurium and Escherichia coli nrdEF operons encoding one of two class I ribonucleotide reductases present in both bacteria.
Jordan A, Aragall E, Gibert I, Barbe J
Mol Microbiol 1996 Feb;19(4):777-90
PubMed ID: 8820648
Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis.
Stephens RS, Kalman S, Lammel C, Fan J, Marathe R, Aravind L, Mitchell W, Olinger L, Tatusov RL, Zhao Q, Koonin EV, Davis RW
Science 1998 Oct 23;282(5389):754-9
PubMed ID: 9784136
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344