Pseudomonas aeruginosa PAO1, PA1498 (pykF)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: pyk-I
Replicon chromosome
Genomic location 1626062 - 1627495 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_250189.1
GI 15596695
Affymetrix PA1498_pykF_at
Entrez 881682
GenBank AAG04887.1
INSDC AAG04887.1
NCBI Locus Tag PA1498
protein_id(GenBank) gb|AAG04887.1|AE004578_9|gnl|PseudoCAP|PA1498
UniParc UPI00000C53AC
UniProtKB Acc Q9I3L4
UniRef100 UniRef100_Q9I3L4
UniRef50 UniRef50_Q44473
UniRef90 UniRef90_Q9I3L4


Feature Type CDS
Coding Frame 1
Product Name
pyruvate kinase I
Product Name Confidence: Class 2
Synonyms Phosphoenolpyruvate kinase
Evidence for Translation
Charge (pH 7) -6.41
Kyte-Doolittle Hydrophobicity Value -0.048
Molecular Weight (kDa) 51.5
Isoelectric Point (pI) 5.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7OO1 TRANSFERASE 05/26/21 Structure, function and characterization of a second pyruvate kinase isozyme in Pseudomonas aeruginosa. Pseudomonas aeruginosa PAO1 3.01 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 614 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001412 (474 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1498
Search term: pykF
Search term: pyruvate kinase I

Human Homologs

Ensembl 110, assembly GRCh38.p14
pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021]
E-value: 1.3e-78
Percent Identity: 39.6
Ensembl 110, assembly GRCh38.p14
pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021]
E-value: 1.3e-78
Percent Identity: 39.6


Primary structure of three peptides at the catalytic and allosteric sites of the fructose-1,6-bisphosphate-activated pyruvate kinase from Escherichia coli.
Speranza ML, Valentini G, Iadarola P, Stoppini M, Malcovati M, Ferri G
Biol Chem Hoppe Seyler 1989 Mar;370(3):211-6
PubMed ID: 2653362
Key residues in the allosteric transition of Bacillus stearothermophilus pyruvate kinase identified by site-directed mutagenesis.
Walker D, Chia WN, Muirhead H
J Mol Biol 1992 Nov 5;228(1):265-76
PubMed ID: 1447787
Crystal structure of Escherichia coli pyruvate kinase type I: molecular basis of the allosteric transition.
Mattevi A, Valentini G, Rizzi M, Speranza ML, Bolognesi M, Coda A
Structure 1995 Jul 15;3(7):729-41
PubMed ID: 8591049