Pseudomonas aeruginosa PAO1, PA1546 (hemN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1546
Name
hemN
Replicon chromosome
Genomic location 1682567 - 1683949 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250237.1
GI 15596743
Affymetrix PA1546_hemN_at
Entrez 883053
GenBank AAG04935.1
INSDC AAG04935.1
NCBI Locus Tag PA1546
protein_id(GenBank) gb|AAG04935.1|AE004582_13|gnl|PseudoCAP|PA1546
TIGR NTL03PA01547
UniParc UPI000012C5AA
UniProtKB Acc P77915
UniProtKB ID HEMN_PSEAE
UniRef100 UniRef100_P77915
UniRef50 UniRef50_O34162
UniRef90 UniRef90_P77915

Product

Feature Type CDS
Coding Frame 1
Product Name
oxygen-independent coproporphyrinogen III oxidase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.10
Kyte-Doolittle Hydrophobicity Value -0.367
Molecular Weight (kDa) 52.5
Isoelectric Point (pI) 5.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 580 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003814 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1546
Search term: hemN

Human Homologs

References

Cloning, DNA sequence, and complementation analysis of the Salmonella typhimurium hemN gene encoding a putative oxygen-independent coproporphyrinogen III oxidase.
Xu K, Elliott T
J Bacteriol 1994 Jun;176(11):3196-203
PubMed ID: 8195073