Pseudomonas aeruginosa PAO1, PA1583 (sdhA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1583
Name
sdhA
Replicon chromosome
Genomic location 1721496 - 1723268 (+ strand)

Cross-References

RefSeq NP_250274.1
GI 15596780
Affymetrix PA1583_sdhA_at
Entrez 881934
GenBank AAG04972.1
INSDC AAG04972.1
NCBI Locus Tag PA1583
protein_id(GenBank) gb|AAG04972.1|AE004586_10|gnl|PseudoCAP|PA1583
TIGR NTL03PA01584
UniParc UPI00000C53F2
UniProtKB Acc Q9I3D5
UniProtKB ID Q9I3D5_PSEAE
UniRef100 UniRef100_W0Z3P2
UniRef50 UniRef50_P31040-3
UniRef90 UniRef90_I7A2G6

Product

Feature Type CDS
Coding Frame 1
Product Name
succinate dehydrogenase (A subunit)
Product Name Confidence: Class 2
Synonyms succinate dehydrogenase (flavoprotein subunit)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (63.53 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -5.71
Kyte-Doolittle Hydrophobicity Value -0.180
Molecular Weight (kDa) 63.5
Isoelectric Point (pI) 6.47

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 610 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003780 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1583
Search term: sdhA

Human Homologs

Ensembl 110, assembly GRCh38.p14
succinate dehydrogenase complex flavoprotein subunit A [Source:HGNC Symbol;Acc:HGNC:10680]
E-value: 6.1e-166
Percent Identity: 56.1

References

Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli.
Wood D, Darlison MG, Wilde RJ, Guest JR
Biochem J 1984 Sep 1;222(2):519-34
PubMed ID: 6383359
Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli.
Darlison MG, Guest JR
Biochem J 1984 Oct 15;223(2):507-17
PubMed ID: 6388571
Transcript analysis of the citrate synthase and succinate dehydrogenase genes of Escherichia coli K12.
Wilde RJ, Guest JR
J Gen Microbiol 1986 Dec;132(12):3239-51
PubMed ID: 3309132
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344