Pseudomonas aeruginosa PAO1, PA1588 (sucC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1588
Name
sucC
Replicon chromosome
Genomic location 1730181 - 1731347 (+ strand)
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_250279.1
GI 15596785
Affymetrix PA1588_sucC_at
DNASU PaCD00006726
Entrez 882016
GenBank AAG04977.1
INSDC CAA58870.1
NCBI Locus Tag PA1588
protein_id(GenBank) gb|AAG04977.1|AE004587_1|gnl|PseudoCAP|PA1588
TIGR NTL03PA01589
UniParc UPI00001361A7
UniProtKB Acc P53593
UniProtKB ID SUCC_PSEAE
UniRef100 UniRef100_B7UVD3
UniRef50 UniRef50_Q883Z4
UniRef90 UniRef90_Q883Z4

Product

Feature Type CDS
Coding Frame 1
Product Name
succinyl-CoA synthetase beta chain
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.22
Kyte-Doolittle Hydrophobicity Value -0.065
Molecular Weight (kDa) 41.5
Isoelectric Point (pI) 6.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 535 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003775 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1588
Search term: sucC

Human Homologs

Ensembl 110, assembly GRCh38.p14
succinate-CoA ligase ADP-forming subunit beta [Source:HGNC Symbol;Acc:HGNC:11448]
E-value: 3.4e-96
Percent Identity: 47.8
Ensembl 110, assembly GRCh38.p14
succinate-CoA ligase ADP-forming subunit beta [Source:HGNC Symbol;Acc:HGNC:11448]
E-value: 3.4e-96
Percent Identity: 47.8

References

Primary structure of the succinyl-CoA synthetase of Escherichia coli.
Buck D, Spencer ME, Guest JR
Biochemistry 1985 Oct 22;24(22):6245-52
PubMed ID: 3002435
The crystal structure of succinyl-CoA synthetase from Escherichia coli at 2.5-A resolution.
Wolodko WT, Fraser ME, James MN, Bridger WA
J Biol Chem 1994 Apr 8;269(14):10883-90
PubMed ID: 8144675
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Succinyl coenzyme A synthetase of Pseudomonas aeruginosa with a broad specificity for nucleoside triphosphate (NTP) synthesis modulates specificity for NTP synthesis by the 12-kilodalton form of nucleoside diphosphate kinase.
Kapatral V, Bina X, Chakrabarty AM
J Bacteriol 2000 Mar;182(5):1333-9
PubMed ID: 10671455