Pseudomonas aeruginosa PAO1, PA1642 (selD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1642
Name
selD
Replicon chromosome
Genomic location 1787250 - 1788284 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250333.1
GI 15596839
Affymetrix PA1642_selD_at
Entrez 877775
GenBank AAG05031.1
INSDC AAG05031.1
NCBI Locus Tag PA1642
protein_id(GenBank) gb|AAG05031.1|AE004592_2|gnl|PseudoCAP|PA1642
TIGR NTL03PA01643
UniParc UPI0000135831
UniProtKB Acc Q9I383
UniProtKB ID SELD_PSEAE
UniRef100 UniRef100_Q9I383
UniRef50 UniRef50_Q9I383
UniRef90 UniRef90_Q9I383

Product

Feature Type CDS
Coding Frame 1
Product Name
selenophosphate synthetase
Product Name Confidence: Class 2
Synonyms selenide,water dikinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.48
Kyte-Doolittle Hydrophobicity Value 0.183
Molecular Weight (kDa) 36.4
Isoelectric Point (pI) 4.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 317 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003732 (525 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1642
Search term: selD

Human Homologs

Ensembl 110, assembly GRCh38.p14
selenophosphate synthetase 2 [Source:HGNC Symbol;Acc:HGNC:19686]
E-value: 2.6e-23
Percent Identity: 28.8

References

In vitro synthesis of selenocysteinyl-tRNA(UCA) from seryl-tRNA(UCA): involvement and characterization of the selD gene product.
Leinfelder W, Forchhammer K, Veprek B, Zehelein E, Böck A
Proc Natl Acad Sci U S A 1990 Jan;87(2):543-7
PubMed ID: 2405383
Biochemical analysis of Escherichia coli selenophosphate synthetase mutants. Lysine 20 is essential for catalytic activity and cysteine 17/19 for 8-azido-ATP derivatization.
Kim IY, Veres Z, Stadtman TC
J Biol Chem 1993 Dec 25;268(36):27020-5
PubMed ID: 8262938