Pseudomonas aeruginosa PAO1, PA1659 (hsiF2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1659
Name
hsiF2
Synonym: tssE2
Replicon chromosome
Genomic location 1807241 - 1807648 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentHcp Secretion Island-II-encoded type VI secretion system (H2-T6SS)

Cross-References

RefSeq NP_250350.1
GI 15596856
Affymetrix PA1659_at
Entrez 880734
GenBank AAG05048.1
INSDC AAG05048.1
NCBI Locus Tag PA1659
protein_id(GenBank) gb|AAG05048.1|AE004593_10|gnl|PseudoCAP|PA1659
TIGR NTL03PA01660
UniParc UPI00000C5431
UniProtKB Acc Q9I366
UniProtKB ID Q9I366_PSEAE
UniRef100 UniRef100_Q9I366
UniRef50 UniRef50_Q9I366
UniRef90 UniRef90_Q9I366

Product

Feature Type CDS
Coding Frame 1
Product Name
HsiF2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -2.56
Kyte-Doolittle Hydrophobicity Value -0.187
Molecular Weight (kDa) 14.7
Isoelectric Point (pI) 5.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000317
genomic context evidence used in manual assertion
Host Organism Homo sapiens
Notes H2-T6SS rendered inactive via clpV2 deletion.
Reference 22665491

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 48 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000086 (815 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1659
Search term: hsiF2
Search term: HsiF2

Human Homologs

References

Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa.
Lossi NS, Dajani R, Freemont P, Filloux A
Microbiology (Reading) 2011 Dec;157(Pt 12):3292-3305
PubMed ID: 21873404
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A
Proc Natl Acad Sci U S A 2017 Jul 18;114(29):7707-7712
PubMed ID: 28673999