Pseudomonas aeruginosa PAO1, PA1726 (bglX)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1726
Name
bglX
Replicon chromosome
Genomic location 1866241 - 1868535 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_250417.1
GI 15596923
Affymetrix PA1726_bglX_at
Entrez 877660
GenBank AAG05115.1
INSDC AAG05115.1
NCBI Locus Tag PA1726
protein_id(GenBank) gb|AAG05115.1|AE004598_14|gnl|PseudoCAP|PA1726
TIGR NTL03PA01727
UniParc UPI00000C5463
UniProtKB Acc Q9I311
UniProtKB ID Q9I311_PSEAE
UniRef100 UniRef100_W0YNV9
UniRef50 UniRef50_P33363
UniRef90 UniRef90_A6V799

Product

Feature Type CDS
Coding Frame 1
Product Name
BglX
Product Name Confidence: Class 1
Synonyms cellobiase
gentobiase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 0.34
Kyte-Doolittle Hydrophobicity Value -0.206
Molecular Weight (kDa) 83.0
Isoelectric Point (pI) 7.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6R5I HYDROLASE 03/25/19 The crystal structure of the Glycoside Hydrolase BglX from P. aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.8 X-RAY DIFFRACTION 100.0
6R5U HYDROLASE 03/25/19 The crystal structure of Glycoside Hydrolase BglX inactive mutant from P. aeruginosa in complex with laminaritriose Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.15 X-RAY DIFFRACTION 99.9
6R5R HYDROLASE 03/25/19 The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with cellobiose Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.65 X-RAY DIFFRACTION 99.9
6R5N HYDROLASE 03/25/19 The crystal structure of Glycoside Hydrolase BglX from P. aeruginosa in complex with 1-deoxynojirimycin Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2 X-RAY DIFFRACTION 100.0
6R5O HYDROLASE 03/25/19 The crystal structure the Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with two glucose molecules Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.4 X-RAY DIFFRACTION 99.9
6R5P HYDROLASE 03/25/19 The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with glucose Pseudomonas aeruginosa PAO1 2.85 X-RAY DIFFRACTION 99.9
6R5T HYDROLASE 03/25/19 The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with lactose Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.6 X-RAY DIFFRACTION 99.9
6R5V HYDROLASE 03/25/19 The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with xylotriose Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.8 X-RAY DIFFRACTION 99.9

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 371 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003663 (520 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1726
Search term: bglX
Search term: BglX

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.
Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S
Microbiology (Reading) 1996 Jul;142 ( Pt 7):1659-65
PubMed ID: 8757730
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Catalytic Cycle of Glycoside Hydrolase BglX from Pseudomonas aeruginosa and Its Implications for Biofilm Formation.
Mahasenan KV, Batuecas MT, De Benedetti S, Kim C, Rana N, Lee M, Hesek D, Fisher JF, Sanz-Aparicio J, Hermoso JA, Mobashery S
ACS Chem Biol 2020 Jan 17;15(1):189-196
PubMed ID: 31877028