Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1756
|
Name |
cysH
|
Replicon | chromosome |
Genomic location | 1895692 - 1896495 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_250447.1 |
GI | 15596953 |
Affymetrix | PA1756_cysH_at |
DNASU | PaCD00006801 |
Entrez | 878560 |
GenBank | AAG05145.1 |
INSDC | AAG05145.1 |
NCBI Locus Tag | PA1756 |
protein_id(GenBank) | gb|AAG05145.1|AE004601_10|gnl|PseudoCAP|PA1756 |
TIGR | NTL03PA01757 |
UniParc | UPI0000128CED |
UniProtKB Acc | O05927 |
UniProtKB ID | CYSH_PSEAE |
UniRef100 | UniRef100_O05927 |
UniRef50 | UniRef50_O05927 |
UniRef90 | UniRef90_O05927 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
3'-phosphoadenosine-5'-phosphosulfate reductase
|
Synonyms |
PAPS reductase |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -2.98 |
Kyte-Doolittle Hydrophobicity Value | -0.447 |
Molecular Weight (kDa) | 30.2 |
Isoelectric Point (pI) | 6.42 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2GOY | OXIDOREDUCTASE | 04/14/06 | Crystal structure of assimilatory adenosine 5'-phosphosulfate reductase with bound APS | Pseudomonas aeruginosa | 2.7 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 386 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003637 (520 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1756
Search term: cysH
Search term: 3'-phosphoadenosine-5'-phosphosulfate reductase
|
Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase.
Ostrowski J, Wu JY, Rueger DC, Miller BE, Siegel LM, Kredich NM
J Biol Chem 1989 Sep 15;264(26):15726-37
PubMed ID: 2670946
|
Three members of a novel small gene-family from Arabidopsis thaliana able to complement functionally an Escherichia coli mutant defective in PAPS reductase activity encode proteins with a thioredoxin-like domain and "APS reductase" activity.
Gutierrez-Marcos JF, Roberts MA, Campbell EI, Wray JL
Proc Natl Acad Sci U S A 1996 Nov 12;93(23):13377-82
PubMed ID: 8917599
|