Pseudomonas aeruginosa PAO1, PA1756 (cysH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1756
Name
cysH
Replicon chromosome
Genomic location 1895692 - 1896495 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250447.1
GI 15596953
Affymetrix PA1756_cysH_at
DNASU PaCD00006801
Entrez 878560
GenBank AAG05145.1
INSDC AAG05145.1
NCBI Locus Tag PA1756
protein_id(GenBank) gb|AAG05145.1|AE004601_10|gnl|PseudoCAP|PA1756
TIGR NTL03PA01757
UniParc UPI0000128CED
UniProtKB Acc O05927
UniProtKB ID CYSH_PSEAE
UniRef100 UniRef100_O05927
UniRef50 UniRef50_O05927
UniRef90 UniRef90_O05927

Product

Feature Type CDS
Coding Frame 1
Product Name
3'-phosphoadenosine-5'-phosphosulfate reductase
Product Name Confidence: Class 2
Synonyms PAPS reductase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.98
Kyte-Doolittle Hydrophobicity Value -0.447
Molecular Weight (kDa) 30.2
Isoelectric Point (pI) 6.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2GOY OXIDOREDUCTASE 04/14/06 Crystal structure of assimilatory adenosine 5'-phosphosulfate reductase with bound APS Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 386 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003637 (520 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1756
Search term: cysH

Human Homologs

References

Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase.
Ostrowski J, Wu JY, Rueger DC, Miller BE, Siegel LM, Kredich NM
J Biol Chem 1989 Sep 15;264(26):15726-37
PubMed ID: 2670946
Three members of a novel small gene-family from Arabidopsis thaliana able to complement functionally an Escherichia coli mutant defective in PAPS reductase activity encode proteins with a thioredoxin-like domain and "APS reductase" activity.
Gutierrez-Marcos JF, Roberts MA, Campbell EI, Wray JL
Proc Natl Acad Sci U S A 1996 Nov 12;93(23):13377-82
PubMed ID: 8917599