Pseudomonas aeruginosa PAO1, PA1796 (folD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1796
Name
folD
Replicon chromosome
Genomic location 1946187 - 1947041 (- strand)

Cross-References

RefSeq NP_250487.1
GI 15596993
Affymetrix PA1796_folD_at
DNASU PaCD00006818
Entrez 880636
GenBank AAG05185.1
INSDC AAG05185.1
NCBI Locus Tag PA1796
protein_id(GenBank) gb|AAG05185.1|AE004605_7|gnl|PseudoCAP|PA1796
TIGR NTL03PA01797
UniParc UPI00000C54A0
UniProtKB Acc Q9I2U6
UniProtKB ID FOLD_PSEAE
UniRef100 UniRef100_B7VB83
UniRef50 UniRef50_P51696
UniRef90 UniRef90_A6V726

Product

Feature Type CDS
Coding Frame 1
Product Name
5,10-methylene-tetrahydrofolate dehydrogenase / cyclohydrolase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.96
Kyte-Doolittle Hydrophobicity Value 0.053
Molecular Weight (kDa) 30.5
Isoelectric Point (pI) 5.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4A5O OXIDOREDUCTASE 10/26/11 Crystal structure of Pseudomonas aeruginosa N5, N10- methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) PSEUDOMONAS AERUGINOSA PAO1 2.2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 621 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003602 (554 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1796
Search term: folD

Human Homologs

Ensembl 97, assembly GRCh38.p12
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase [Source:HGNC Symbol;Acc:HGNC:7434]
E-value: 1.0e-58
Percent Identity: 43.7
Ensembl 97, assembly GRCh38.p12
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 like [Source:HGNC Symbol;Acc:HGNC:31865]
E-value: 1.0e-58
Percent Identity: 43.7

References

Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from Escherichia coli.
D'Ari L, Rabinowitz JC
J Biol Chem 1991 Dec 15;266(35):23953-8
PubMed ID: 1748668
fimA-folD genes linkage in Salmonella identifies a putative junctional site of chromosomal rearrangement in the enterobacterial genome.
Rossolini GM, Muscas P, Chiesurin A, Satta G
FEMS Microbiol. Lett. 1994 Jun;119(3):321-8
PubMed ID: 8050713
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994