Pseudomonas aeruginosa PAO1, PA1845 (tsi1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1845
Name
tsi1
Replicon chromosome
Genomic location 2004374 - 2004892 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250536.1
GI 15597042
Affymetrix PA1845_at
DNASU PaCD00006834
Entrez 880643
GenBank AAG05234.1
INSDC AAG05234.1
NCBI Locus Tag PA1845
protein_id(GenBank) gb|AAG05234.1|AE004610_7|gnl|PseudoCAP|PA1845
TIGR NTL03PA01846
UniParc UPI00000C54C9
UniProtKB Acc Q9I2Q0
UniProtKB ID Q9I2Q0_PSEAE
UniRef100 UniRef100_W0YR21
UniRef50 UniRef50_A6V6X2
UniRef90 UniRef90_A6V6X2

Product

Feature Type CDS
Coding Frame 1
Product Name
Tsi1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -7.46
Kyte-Doolittle Hydrophobicity Value -0.355
Molecular Weight (kDa) 18.8
Isoelectric Point (pI) 4.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4FQB TOXIN/IMMUNE SYSTEM 06/25/12 crystal structure of toxic effector Tse1 in complex with immune protein Tsi1 Pseudomonas aeruginosa PAO1 2.69 X-RAY DIFFRACTION 100.0
3VPJ HYDROLASE/HYDROLASE INHIBITOR 03/04/12 crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa in complex with immune protein Tsi1 Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 100.0
4EQA UNKNOWN FUNCTION 04/18/12 Crystal structure of PA1844 in complex with PA1845 from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa 1.6 X-RAY DIFFRACTION 100.0
4FGI HYDROLASE/HYDROLASE INHIBITOR 06/04/12 Structure of the effector - immunity system Tse1 / Tsi1 from Pseudomonas aeruginosa Pseudomonas aeruginosa 3.2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003555 (300 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1845
Search term: tsi1
Search term: Tsi1

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Type VI secretion delivers bacteriolytic effectors to target cells.
Russell AB, Hood RD, Bui NK, LeRoux M, Vollmer W, Mougous JD
Nature 2011 Jul 20;475(7356):343-7
PubMed ID: 21776080