Pseudomonas aeruginosa PAO1, PA2052 (cynS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2052
Name
cynS
Replicon chromosome
Genomic location 2245986 - 2246456 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_250742.1
GI 15597248
Affymetrix PA2052_cynS_at
Entrez 877801
GenBank AAG05440.1
INSDC AAG05440.1
NCBI Locus Tag PA2052
protein_id(GenBank) gb|AAG05440.1|AE004631_9|gnl|PseudoCAP|PA2052
TIGR NTL03PA02052
UniParc UPI0000128C40
UniProtKB Acc Q9I263
UniProtKB ID CYNS_PSEAE
UniRef100 UniRef100_B7VA51
UniRef50 UniRef50_P58704
UniRef90 UniRef90_A6V6B1

Product

Feature Type CDS
Coding Frame 1
Product Name
cyanate lyase
Product Name Confidence: Class 2
Synonyms cyanase
cyanate hydrolase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.29
Kyte-Doolittle Hydrophobicity Value 0.040
Molecular Weight (kDa) 16.8
Isoelectric Point (pI) 6.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 113 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003395 (437 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2052
Search term: cynS
Search term: cyanate lyase

Human Homologs

References

The amino acid sequence of Escherichia coli cyanase.
Chin CC, Anderson PM, Wold F
J Biol Chem 1983 Jan 10;258(1):276-82
PubMed ID: 6336748
Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase.
Sung YC, Anderson PM, Fuchs JA
J Bacteriol 1987 Nov;169(11):5224-30
PubMed ID: 2822670
Characterization of the cyn operon in Escherichia coli K12.
Sung YC, Fuchs JA
J Biol Chem 1988 Oct 15;263(29):14769-75
PubMed ID: 3049588
The cyanase operon and cyanate metabolism.
Anderson PM, Sung YC, Fuchs JA
FEMS Microbiol Rev 1990 Dec;7(3-4):247-52
PubMed ID: 2094285