Pseudomonas aeruginosa PAO1, PA2626 (trmU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2626
Name
trmU
Synonym: asuE
Replicon chromosome
Genomic location 2969987 - 2971114 (+ strand)

Cross-References

RefSeq NP_251316.1
GI 15597822
Affymetrix PA2626_trmU_at
Entrez 882332
GenBank AAG06014.1
INSDC AAG06014.1
NCBI Locus Tag PA2626
protein_id(GenBank) gb|AAG06014.1|AE004691_5|gnl|PseudoCAP|PA2626
TIGR NTL03PA02626
UniParc UPI0000137485
UniProtKB Acc Q9I0L2
UniProtKB ID MNMA_PSEAE
UniRef100 UniRef100_Q02NB8
UniRef50 UniRef50_Q8X735
UniRef90 UniRef90_A6V4G0

Product

Feature Type CDS
Coding Frame 1
Product Name
tRNA methyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.24
Kyte-Doolittle Hydrophobicity Value -0.419
Molecular Weight (kDa) 41.9
Isoelectric Point (pI) 5.92

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 582 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002926 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2626
Search term: trmU

Human Homologs

Ensembl 110, assembly GRCh38.p14
tRNA mitochondrial 2-thiouridylase [Source:HGNC Symbol;Acc:HGNC:25481]
E-value: 1.1e-75
Percent Identity: 42.0

References

Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR.
He B, Smith JM, Zalkin H
J Bacteriol 1992 Jan;174(1):130-6
PubMed ID: 1729205
Reduced misreading of asparagine codons by Escherichia coli tRNALys with hypomodified derivatives of 5-methylaminomethyl-2-thiouridine in the wobble position.
Hagervall TG, Pomerantz SC, McCloskey JA
J Mol Biol 1998 Nov 20;284(1):33-42
PubMed ID: 9811540