Pseudomonas aeruginosa PAO1, PA2962 (tmk)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA2962
Name	tmk
Replicon	chromosome
Genomic location	3321043 - 3321675 (- strand)

Cross-References

RefSeq	NP_251652.1
GI	15598158
Affymetrix	PA2962_tmk_at
DNASU	PaCD00007293
Entrez	878730
GenBank	AAG06350.1
INSDC	AAG06350.1
NCBI Locus Tag	PA2962
protein_id(GenBank)	gb|AAG06350.1|AE004722_6|gnl|PseudoCAP|PA2962
TIGR	NTL03PA02962
UniParc	UPI000012E0FF
UniProtKB Acc	Q9HZN8
UniProtKB ID	KTHY_PSEAE
UniRef100	UniRef100_B7V167
UniRef50	UniRef50_B7V167
UniRef90	UniRef90_B7V167

Product

Feature Type	CDS
Coding Frame	1
Product Name	thymidylate kinase Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-4.79
Kyte-Doolittle Hydrophobicity Value	-0.177
Molecular Weight (kDa)	23.1
Isoelectric Point (pI)	4.86

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3UWK	TRANSFERASE/TRANSFERASE INHIBITOR	12/02/11	Structure Guided Development of Novel Thymidine Mimetics targeting Pseudomonas aeruginosa Thymidylate Kinase: from Hit to Lead Generation	Pseudomonas aeruginosa	1.91	X-RAY DIFFRACTION	100.0
4EDH	TRANSFERASE	03/27/12	The crystal structure of thymidylate kinase from Pseudomonas aeruginosa PAO1 in complex with ADP,TMP and Mg.	Pseudomonas aeruginosa PAO1	1.32	X-RAY DIFFRACTION	100.0
3UWO	TRANSFERASE/TRANSFERASE INHIBITOR	12/02/11	Structure Guided Development of Novel Thymidine Mimetics targeting Pseudomonas aeruginosa Thymidylate Kinase: from Hit to Lead Generation	Pseudomonas aeruginosa	1.7	X-RAY DIFFRACTION	100.0
4ESH	TRANSFERASE	04/23/12	The crystal structure of thymidylate kinase from Pseudomonas aeruginosa PAO1 in complex with deoxythymidine.	Pseudomonas aeruginosa	1.95	X-RAY DIFFRACTION	100.0
4GMD	Transferase/Inhibitor	08/15/12	The crystal structure of thymidylate kinase from Pseudomonas aeruginosa PAO1 in complex with AZT Monophosphate	Pseudomonas aeruginosa	1.98	X-RAY DIFFRACTION	100.0
3UXM	TRANSFERASE/TRANSFERASE INHIBITOR	12/05/11	Structure Guided Development of Novel Thymidine Mimetics targeting Pseudomonas aeruginosa Thymidylate Kinase: from Hit to Lead Generation	Pseudomonas aeruginosa	1.95	X-RAY DIFFRACTION	100.0
4E5U	TRANSFERASE	03/14/12	The crystal structure of thymidylate kinase from Pseudomonas aeruginosa PAO1 in complex with thymidine monophosphate.	Pseudomonas aeruginosa	1.812	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 570 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002620 (530 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA2962 Search term: tmk Search term: thymidylate kinase

Human Homologs

References

Escherichia coli thymidylate kinase: molecular cloning, nucleotide sequence, and genetic organization of the corresponding tmk locus. Reynes JP, Tiraby M, Baron M, Drocourt D, Tiraby G J Bacteriol 1996 May;178(10):2804-12 PubMed ID: 8631667