Pseudomonas aeruginosa PAO1, PA2962 (tmk)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2962
Name
tmk
Replicon chromosome
Genomic location 3321043 - 3321675 (- strand)

Cross-References

RefSeq NP_251652.1
GI 15598158
Affymetrix PA2962_tmk_at
DNASU PaCD00007293
Entrez 878730
GenBank AAG06350.1
INSDC AAG06350.1
NCBI Locus Tag PA2962
protein_id(GenBank) gb|AAG06350.1|AE004722_6|gnl|PseudoCAP|PA2962
TIGR NTL03PA02962
UniParc UPI000012E0FF
UniProtKB Acc Q9HZN8
UniProtKB ID KTHY_PSEAE
UniRef100 UniRef100_B7V167
UniRef50 UniRef50_B7V167
UniRef90 UniRef90_B7V167

Product

Feature Type CDS
Coding Frame 1
Product Name
thymidylate kinase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.79
Kyte-Doolittle Hydrophobicity Value -0.177
Molecular Weight (kDa) 23.1
Isoelectric Point (pI) 4.86

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3UWK TRANSFERASE/TRANSFERASE INHIBITOR 12/02/11 Structure Guided Development of Novel Thymidine Mimetics targeting Pseudomonas aeruginosa Thymidylate Kinase: from Hit to Lead Generation Pseudomonas aeruginosa 1.91 X-RAY DIFFRACTION 100.0
3UXM TRANSFERASE/TRANSFERASE INHIBITOR 12/05/11 Structure Guided Development of Novel Thymidine Mimetics targeting Pseudomonas aeruginosa Thymidylate Kinase: from Hit to Lead Generation Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 100.0
4E5U TRANSFERASE 03/14/12 The crystal structure of thymidylate kinase from Pseudomonas aeruginosa PAO1 in complex with thymidine monophosphate. Pseudomonas aeruginosa 1.812 X-RAY DIFFRACTION 100.0
4EDH TRANSFERASE 03/27/12 The crystal structure of thymidylate kinase from Pseudomonas aeruginosa PAO1 in complex with ADP,TMP and Mg. Pseudomonas aeruginosa PAO1 1.32 X-RAY DIFFRACTION 100.0
4ESH TRANSFERASE 04/23/12 The crystal structure of thymidylate kinase from Pseudomonas aeruginosa PAO1 in complex with deoxythymidine. Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 100.0
3UWO TRANSFERASE/TRANSFERASE INHIBITOR 12/02/11 Structure Guided Development of Novel Thymidine Mimetics targeting Pseudomonas aeruginosa Thymidylate Kinase: from Hit to Lead Generation Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0
4GMD Transferase/Inhibitor 08/15/12 The crystal structure of thymidylate kinase from Pseudomonas aeruginosa PAO1 in complex with AZT Monophosphate Pseudomonas aeruginosa 1.98 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 570 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002620 (530 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2962
Search term: tmk

Human Homologs

References

Escherichia coli thymidylate kinase: molecular cloning, nucleotide sequence, and genetic organization of the corresponding tmk locus.
Reynes JP, Tiraby M, Baron M, Drocourt D, Tiraby G
J Bacteriol 1996 May;178(10):2804-12
PubMed ID: 8631667