Pseudomonas aeruginosa PAO1, PA3004

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3004
Name
Replicon chromosome
Genomic location 3365007 - 3365744 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentDetailed sequence analysis and genetic evidence provided in reference. Listed as 5-methylthioadenosine phosphorylase in NCBI.

Cross-References

RefSeq NP_251694.1
GI 15598200
Affymetrix PA3004_at
DNASU PaCD00007325
Entrez 880215
GenBank AAG06392.1
INSDC AAG06392.1
NCBI Locus Tag PA3004
protein_id(GenBank) gb|AAG06392.1|AE004725_5|gnl|PseudoCAP|PA3004
TIGR NTL03PA03004
UniParc UPI00000C58C2
UniProtKB Acc Q9HZK1
UniProtKB ID MTIP_PSEAE
UniRef100 UniRef100_Q9HZK1
UniRef50 UniRef50_Q9HZK1
UniRef90 UniRef90_Q9HZK1

Product

Feature Type CDS
Coding Frame 1
Product Name
5-methylthioadenosine phosphorylase MtnP
Product Name Confidence: Class 1
Synonyms S-methyl-5'-thioinosine phosphorylase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.73
Kyte-Doolittle Hydrophobicity Value 0.010
Molecular Weight (kDa) 26.3
Isoelectric Point (pI) 6.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3OZB TRANSFERASE 09/24/10 Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine Pseudomonas aeruginosa 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 455 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002578 (423 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3004

Human Homologs

References

Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and annotation of a novel enzyme in quorum sensing.
Guan R, Ho MC, Almo SC, Schramm VL
Biochemistry 2011 Feb 22;50(7):1247-54
PubMed ID: 21197954