Pseudomonas aeruginosa PAO1, PA3005 (nagZ)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3005
Name	nagZ Synonym: ycfO
Replicon	chromosome
Genomic location	3365756 - 3366754 (- strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_251695.1
GI	15598201
Affymetrix	PA3005_at
Entrez	880216
GenBank	AAG06393.1
INSDC	AAG06393.1
NCBI Locus Tag	PA3005
protein_id(GenBank)	gb|AAG06393.1|AE004725_6|gnl|PseudoCAP|PA3005
TIGR	NTL03PA03005
UniParc	UPI000012FD11
UniProtKB Acc	Q9HZK0
UniProtKB ID	NAGZ_PSEAE
UniRef100	UniRef100_B7UYS5
UniRef50	UniRef50_Q8ZFS3
UniRef90	UniRef90_B7UYS5

Product

Feature Type	CDS
Coding Frame	1
Product Name	beta-N-acetyl-D-glucosaminidase Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-4.70
Kyte-Doolittle Hydrophobicity Value	-0.068
Molecular Weight (kDa)	36.1
Isoelectric Point (pI)	6.18

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
5G5K	HYDROLASE	05/25/16	Crystal structure of NagZ from Pseudomonas aeruginosa in complex with the inhibitor 2-acetamido-1,2-dideoxynojirimycin	PSEUDOMONAS AERUGINOSA	3.1	X-RAY DIFFRACTION	100.0
5G2M	HYDROLASE	04/09/16	Crystal structure of NagZ from Pseudomonas aeruginosa in complex with N-acetylglucosamine	PSEUDOMONAS AERUGINOSA	3	X-RAY DIFFRACTION	100.0
5G6T	HYDROLASE	07/15/16	Crystal structure of Zn-containing NagZ H174A mutant from Pseudomonas aeruginosa	PSEUDOMONAS AERUGINOSA	2.15	X-RAY DIFFRACTION	99.7
5G3R	HYDROLASE	04/30/16	Crystal structure of NagZ from Pseudomonas aeruginosa in complex with N-acetylglucosamine and L-Ala-1,6-anhydroMurNAc	PSEUDOMONAS AERUGINOSA	2.18	X-RAY DIFFRACTION	100.0
5G5U	HYDROLASE	06/05/16	Crystal structure of NagZ H174A mutant from Pseudomonas aeruginosa	PSEUDOMONAS AERUGINOSA	2.25	X-RAY DIFFRACTION	99.7
5LY7	GENE REGULATION	09/25/16	Crystal structure of NagZ H174A mutant from Pseudomonas aeruginosa in complex with the inhibitor 2-acetamido-1,2-dideoxynojirimycin	Pseudomonas aeruginosa	3.1	X-RAY DIFFRACTION	99.7
5G1M	HYDROLASE	03/28/16	Crystal structure of NagZ from Pseudomonas aeruginosa	PSEUDOMONAS AERUGINOSA	1.8	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 482 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002577 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3005 Search term: nagZ Search term: beta-N-acetyl-D-glucosaminidase

Human Homologs

References

Molecular characterization of the beta-N-acetylglucosaminidase of Escherichia coli and its role in cell wall recycling. Cheng Q, Li H, Merdek K, Park JT J Bacteriol 2000 Sep;182(17):4836-40 PubMed ID: 10940025

Molecular cloning and characterization of a novel beta-N-acetyl-D-glucosaminidase from Vibrio furnissii. Chitlaru E, Roseman S J Biol Chem 1996 Dec 27;271(52):33433-9 PubMed ID: 8969206

NagZ inactivation prevents and reverts beta-lactam resistance, driven by AmpD and PBP 4 mutations, in Pseudomonas aeruginosa. Zamorano L, Reeve TM, Deng L, Juan C, Moyá B, Cabot G, Vocadlo DJ, Mark BL, Oliver A Antimicrob Agents Chemother 2010 Sep;54(9):3557-63 PubMed ID: 20566764

Inactivation of the glycoside hydrolase NagZ attenuates antipseudomonal beta-lactam resistance in Pseudomonas aeruginosa. Asgarali A, Stubbs KA, Oliver A, Vocadlo DJ, Mark BL Antimicrob Agents Chemother 2009 Jun;53(6):2274-82 PubMed ID: 19273679