Pseudomonas aeruginosa PAO1, PA3011 (topA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3011
Name
topA
Replicon chromosome
Genomic location 3370100 - 3372706 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_251701.1
GI 15598207
Affymetrix PA3011_topA_at
Entrez 880353
GenBank AAG06399.1
INSDC AAG06399.1
NCBI Locus Tag PA3011
protein_id(GenBank) gb|AAG06399.1|AE004726_5|gnl|PseudoCAP|PA3011
TIGR NTL03PA03011
UniParc UPI0000137145
UniProtKB Acc Q9HZJ5
UniProtKB ID TOP1_PSEAE
UniRef100 UniRef100_Q9HZJ5
UniRef50 UniRef50_Q9HZJ5
UniRef90 UniRef90_Q9HZJ5

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA topoisomerase I
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 13.98
Kyte-Doolittle Hydrophobicity Value -0.617
Molecular Weight (kDa) 97.3
Isoelectric Point (pI) 8.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 665 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002571 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3011
Search term: topA

Human Homologs

References

Complete nucleotide sequence of the topA gene encoding Escherichia coli DNA topoisomerase I.
Tse-Dinh YC, Wang JC
J Mol Biol 1986 Oct 5;191(3):321-31
PubMed ID: 3029379
Solution structure of the C-terminal single-stranded DNA-binding domain of Escherichia coli topoisomerase I.
Yu L, Zhu CX, Tse-Dinh YC, Fesik SW
Biochemistry 1995 Jun 13;34(23):7622-8
PubMed ID: 7779808