Pseudomonas aeruginosa PAO1, PA3050 (pyrD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3050
Name
pyrD
Replicon chromosome
Genomic location 3414701 - 3415729 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_251740.1
GI 15598246
Affymetrix PA3050_pyrD_at
DNASU PaCD00007345
Entrez 882867
GenBank AAG06438.1
INSDC AAG06438.1
NCBI Locus Tag PA3050
protein_id(GenBank) gb|AAG06438.1|AE004730_2|gnl|PseudoCAP|PA3050
TIGR NTL03PA03050
UniParc UPI00000C58EA
UniProtKB Acc Q9HZF8
UniProtKB ID PYRD_PSEAE
UniRef100 UniRef100_B7UWL8
UniRef50 UniRef50_B7UWL8
UniRef90 UniRef90_B7UWL8

Product

Feature Type CDS
Coding Frame 1
Product Name
dihydroorotate dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.65
Kyte-Doolittle Hydrophobicity Value 0.027
Molecular Weight (kDa) 36.1
Isoelectric Point (pI) 8.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 595 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002538 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3050
Search term: pyrD

Human Homologs

Ensembl 110, assembly GRCh38.p14
dihydroorotate dehydrogenase (quinone) [Source:HGNC Symbol;Acc:HGNC:2867]
E-value: 2.9e-67
Percent Identity: 44.0

References

Nucleotide sequence of the pyrD gene of Escherichia coli and characterization of the flavoprotein dihydroorotate dehydrogenase.
Larsen JN, Jensen KF
Eur J Biochem 1985 Aug 15;151(1):59-65
PubMed ID: 2992959