Pseudomonas aeruginosa PAO1, PA3099 (xcpV)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3099
Name
xcpV
Synonym: pddC
Replicon chromosome
Genomic location 3479335 - 3479724 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251789.1
GI 15598295
Affymetrix PA3099_xcpV_at
DNASU PaCD00007364
Entrez 882777
GenBank AAG06487.1
INSDC AAG06487.1
NCBI Locus Tag PA3099
protein_id(GenBank) gb|AAG06487.1|AE004734_5|gnl|PseudoCAP|PA3099
TIGR NTL03PA03099
UniParc UPI000012BC52
UniProtKB Acc Q00516
UniProtKB ID GSPI_PSEAE
UniRef100 UniRef100_Q00516
UniRef50 UniRef50_Q00516
UniRef90 UniRef90_Q00516

Product

Feature Type CDS
Coding Frame 1
Product Name
general secretion pathway protein I
Product Name Confidence: Class 1
Synonyms PilD-dependent protein PddC
Evidence for Translation
Charge (pH 7) 2.99
Kyte-Doolittle Hydrophobicity Value -0.240
Molecular Weight (kDa) 14.4
Isoelectric Point (pI) 10.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5VTM TRANSPORT PROTEIN 05/17/17 The crystal structure of minor pseudopilin ternary complex of XcpVWX from the Type 2 secretion system of Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.041 X-RAY DIFFRACTION 100.0
5BW0 PROTEIN TRANSPORT 06/05/15 The crystal structure of minor pseudopilin binary complex of XcpV and XcpW from the Type 2 secretion system of Pseudomonas aeruginosa Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0
6UTU PROTEIN TRANSPORT 10/30/19 Crystal structure of minor pseudopilin ternary complex of XcpVWX from the Type 2 secretion system of Pseudomonas aeruginosa in the P3 space group Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.85 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 92 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002495 (370 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3099
Search term: xcpV

Human Homologs

References

Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -W.
Nunn DN, Lory S
J Bacteriol 1993 Jul;175(14):4375-82
PubMed ID: 8331069
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Components of the protein-excretion apparatus of Pseudomonas aeruginosa are processed by the type IV prepilin peptidase.
Nunn DN, Lory S
Proc Natl Acad Sci U S A 1992 Jan 1;89(1):47-51
PubMed ID: 1309616
Protein secretion in Pseudomonas aeruginosa: characterization of seven xcp genes and processing of secretory apparatus components by prepilin peptidase.
Bally M, Filloux A, Akrim M, Ball G, Lazdunski A, Tommassen J
Mol Microbiol 1992 May;6(9):1121-31
PubMed ID: 1588814