Pseudomonas aeruginosa PAO1, PA3101 (xcpT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3101
Name
xcpT
Synonym: pddA
Replicon chromosome
Genomic location 3480246 - 3480692 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251791.1
GI 15598297
Affymetrix PA3101_xcpT_at
DNASU PaCD00007365
Entrez 878731
GenBank AAG06489.1
INSDC AAG06489.1
NCBI Locus Tag PA3101
protein_id(GenBank) gb|AAG06489.1|AE004734_7|gnl|PseudoCAP|PA3101
TIGR NTL03PA03101
UniParc UPI000012BC42
UniProtKB Acc Q00514
UniProtKB ID GSPG_PSEAE
UniRef100 UniRef100_Q00514
UniRef50 UniRef50_Q00514
UniRef90 UniRef90_Q00514

Product

Feature Type CDS
Coding Frame 1
Product Name
general secretion pathway protein G
Product Name Confidence: Class 1
Synonyms PilD-dependent protein PddA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.98
Kyte-Doolittle Hydrophobicity Value -0.370
Molecular Weight (kDa) 16.1
Isoelectric Point (pI) 8.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2KEP TRANSPORT PROTEIN 02/02/09 Solution structure of XcpT, the main component of the type 2 secretion system of Pseudomonas aeruginosa Pseudomonas aeruginosa NOT SOLUTION NMR 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 278 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000660 (797 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3101
Search term: xcpT

Human Homologs

References

Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -W.
Nunn DN, Lory S
J Bacteriol 1993 Jul;175(14):4375-82
PubMed ID: 8331069
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Components of the protein-excretion apparatus of Pseudomonas aeruginosa are processed by the type IV prepilin peptidase.
Nunn DN, Lory S
Proc Natl Acad Sci U S A 1992 Jan 1;89(1):47-51
PubMed ID: 1309616
Protein secretion in Pseudomonas aeruginosa: characterization of seven xcp genes and processing of secretory apparatus components by prepilin peptidase.
Bally M, Filloux A, Akrim M, Ball G, Lazdunski A, Tommassen J
Mol Microbiol 1992 May;6(9):1121-31
PubMed ID: 1588814