Pseudomonas aeruginosa PAO1, PA3105 (xcpQ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3105
Name
xcpQ
Replicon chromosome
Genomic location 3484354 - 3486330 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq NP_251795.1
GI 15598301
Affymetrix PA3105_xcpQ_at
Entrez 880114
GenBank AAG06493.1
INSDC AAG06493.1
NCBI Locus Tag PA3105
protein_id(GenBank) gb|AAG06493.1|AE004734_11|gnl|PseudoCAP|PA3105
TIGR NTL03PA03105
UniParc UPI000012BC2A
UniProtKB Acc P35818
UniProtKB ID GSPD_PSEAE
UniRef100 UniRef100_P35818
UniRef50 UniRef50_P35818
UniRef90 UniRef90_P35818

Product

Feature Type CDS
Coding Frame 1
Product Name
general secretion pathway protein D
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.04
Kyte-Doolittle Hydrophobicity Value -0.123
Molecular Weight (kDa) 70.0
Isoelectric Point (pI) 5.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5MP2 TRANSPORT PROTEIN 12/15/16 XcpQN012 in complex with VHH04 Lama glama; Pseudomonas aeruginosa 2.9 X-RAY DIFFRACTION 100.0
4E9J PROTEIN TRANSPORT 03/21/12 Crystal structure of the N-terminal domain of the secretin XcpQ from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.03 X-RAY DIFFRACTION 99.2
5NGI TRANSPORT PROTEIN 03/17/17 Structure of XcpQN012 Pseudomonas aeruginosa 2.98 X-RAY DIFFRACTION 100.0
4EC5 PROTEIN TRANSPORT 03/26/12 Crystal structure of the S210C (dimer) mutant from the N-terminal domain of the secretin XcpQ from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.197 X-RAY DIFFRACTION 98.8
5WLN MEMBRANE PROTEIN 07/27/17 Cryo-EM structure of the T2SS secretin XcpQ from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.57 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 348 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000664 (1798 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3105
Search term: xcpQ

Human Homologs

References

Xcp-mediated protein secretion in Pseudomonas aeruginosa: identification of two additional genes and evidence for regulation of xcp gene expression.
Akrim M, Bally M, Ball G, Tommassen J, Teerink H, Filloux A, Lazdunski A
Mol Microbiol 1993 Oct;10(2):431-43
PubMed ID: 7934833
Formation of oligomeric rings by XcpQ and PilQ, which are involved in protein transport across the outer membrane of Pseudomonas aeruginosa.
Bitter W, Koster M, Latijnhouwers M, de Cock H, Tommassen J
Mol Microbiol 1998 Jan;27(1):209-19
PubMed ID: 9466268
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994