Pseudomonas aeruginosa PAO1, PA3280 (oprO)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3280
Name
oprO
Replicon chromosome
Genomic location 3673008 - 3674324 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251970.1
GI 15598476
Affymetrix PA3280_oprO_at
Entrez 882443
GenBank AAG06668.1
INSDC AAG06668.1
NCBI Locus Tag PA3280
protein_id(GenBank) gb|AAG06668.1|AE004750_6|gnl|PseudoCAP|PA3280
TIGR NTL03PA03280
UniParc UPI0000131F7E
UniProtKB Acc P32977
UniProtKB ID PORO_PSEAE
UniRef100 UniRef100_P32977
UniRef50 UniRef50_P05695
UniRef90 UniRef90_P32977

Product

Feature Type CDS
Coding Frame 1
Product Name
Pyrophosphate-specific outer membrane porin OprO precursor
Product Name Confidence: Class 1
Synonyms porin O
Evidence for Translation
Charge (pH 7) -9.55
Kyte-Doolittle Hydrophobicity Value -0.499
Molecular Weight (kDa) 47.8
Isoelectric Point (pI) 4.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4RJW MEMBRANE PROTEIN 10/11/14 Crystal structure of Pseudomonas aeruginosa OprO Pseudomonas aeruginosa 1.52 X-RAY DIFFRACTION 100.0
4RJX MEMBRANE PROTEIN 10/11/14 Crystal structure of the OprO mutant protein F62Y/D114Y Pseudomonas aeruginosa 1.54 X-RAY DIFFRACTION 99.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 45 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002334 (687 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3280
Search term: oprO

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Polyphosphate-selective porin OprO of Pseudomonas aeruginosa: expression, purification and sequence.
Siehnel RJ, Egli C, Hancock RE
Mol Microbiol 1992 Aug;6(16):2319-26
PubMed ID: 1406271