Pseudomonas aeruginosa PAO1, PA3300 (fadD2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3300
Name
fadD2
Replicon chromosome
Genomic location 3697434 - 3699122 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_251990.1
GI 15598496
Affymetrix PA3300_fadD2_at
Entrez 882465
GenBank AAG06688.1
INSDC AAG06688.1
NCBI Locus Tag PA3300
protein_id(GenBank) gb|AAG06688.1|AE004752_4|gnl|PseudoCAP|PA3300
TIGR NTL03PA03300
UniParc UPI00000C599E
UniProtKB Acc Q9HYU3
UniProtKB ID Q9HYU3_PSEAE
UniRef100 UniRef100_W0YST4
UniRef50 UniRef50_P69452
UniRef90 UniRef90_A0A080VPF0

Product

Feature Type CDS
Coding Frame 1
Product Name
long-chain-fatty-acid--CoA ligase
Product Name Confidence: Class 2
Synonyms acyl coenzyme A synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.70
Kyte-Doolittle Hydrophobicity Value -0.012
Molecular Weight (kDa) 61.7
Isoelectric Point (pI) 7.18

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 603 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002317 (1059 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3300
Search term: fadD2

Human Homologs

Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase family member 3 [Source:HGNC Symbol;Acc:HGNC:27288]
E-value: 4.2e-39
Percent Identity: 29.9
Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase family member 3 [Source:HGNC Symbol;Acc:HGNC:27288]
E-value: 4.2e-39
Percent Identity: 29.9
Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase family member 3 [Source:HGNC Symbol;Acc:HGNC:27288]
E-value: 4.2e-39
Percent Identity: 29.9

References

Multiple FadD acyl-CoA synthetases contribute to differential fatty acid degradation and virulence in Pseudomonas aeruginosa.
Kang Y, Zarzycki-Siek J, Walton CB, Norris MH, Hoang TT
PLoS One 2010 Oct 21;5(10):e13557
PubMed ID: 21042406