Pseudomonas aeruginosa PAO1, PA3366 (amiE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3366
Name
amiE
Replicon chromosome
Genomic location 3776959 - 3777999 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252056.1
GI 15598562
Affymetrix PA3366_amiE_at
DNASU PaCD00007475
Entrez 877816
GenBank AAG06754.1
INSDC AAG06754.1
NCBI Locus Tag PA3366
protein_id(GenBank) gb|AAG06754.1|AE004759_1|gnl|PseudoCAP|PA3366
TIGR NTL03PA03366
UniParc UPI00001257AB
UniProtKB Acc P11436
UniProtKB ID AMIE_PSEAE
UniRef100 UniRef100_P11436
UniRef50 UniRef50_P11436
UniRef90 UniRef90_P11436

Product

Feature Type CDS
Coding Frame 1
Product Name
aliphatic amidase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.59
Kyte-Doolittle Hydrophobicity Value -0.330
Molecular Weight (kDa) 38.5
Isoelectric Point (pI) 5.14

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2UXY HYDROLASE 04/02/07 Aliphatic amidase PSEUDOMONAS AERUGINOSA 1.25 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 371 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002262 (359 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3366
Search term: amiE
Search term: aliphatic amidase

Human Homologs

References

Kinetic mechanism of the aliphatic amidase from Pseudomonas aeruginosa.
Woods MJ, Findlater JD, Orsi BA
Biochim Biophys Acta 1979 Mar 16;567(1):225-37
PubMed ID: 110350
The amino acid sequence of the aliphatic amidase from Pseudomonas aeruginosa.
Ambler RP, Auffret AD, Clarke PH
FEBS Lett 1987 May 11;215(2):285-90
PubMed ID: 3108029
The nucleotide sequence of the amiE gene of Pseudomonas aeruginosa.
Brammar WJ, Charles IG, Matfield M, Liu CP, Drew RE, Clarke PH
FEBS Lett 1987 May 11;215(2):291-4
PubMed ID: 3108030