Pseudomonas aeruginosa PAO1, PA3475 (pheC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3475
Name
pheC
Replicon chromosome
Genomic location 3888178 - 3888984 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252165.1
GI 15598671
Affymetrix PA3475_pheC_at
Entrez 878976
GenBank AAG06863.1
INSDC AAG06863.1
NCBI Locus Tag PA3475
protein_id(GenBank) gb|AAG06863.1|AE004768_8|gnl|PseudoCAP|PA3475
TIGR NTL03PA03475
UniParc UPI0000131955
UniProtKB Acc Q01269
UniProtKB ID PHEC_PSEAE
UniRef100 UniRef100_Q01269
UniRef50 UniRef50_Q01269
UniRef90 UniRef90_Q01269

Product

Feature Type CDS
Coding Frame 1
Product Name
cyclohexadienyl dehydratase precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -0.41
Kyte-Doolittle Hydrophobicity Value -0.331
Molecular Weight (kDa) 30.4
Isoelectric Point (pI) 6.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3KBR LYASE 10/20/09 The crystal structure of cyclohexadienyl dehydratase precursor from Pseudomonas aeruginosa PA01 Pseudomonas aeruginosa 1.659 X-RAY DIFFRACTION 100.0
5HPQ LYASE 01/20/16 Crystal structure of cyclohexadienyl dehydratase from Pseudomonas aeruginosa bound to acetate Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.05 X-RAY DIFFRACTION 100.0
6BQE LYASE 11/27/17 Low-resolution structure of cyclohexadienyl dehydratase from Pseudomonas aeruginosa in space group P4322. Pseudomonas aeruginosa 3.2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 391 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002166 (381 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3475
Search term: pheC

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cyclohexadienyl dehydratase from Pseudomonas aeruginosa. Molecular cloning of the gene and characterization of the gene product.
Zhao GS, Xia TH, Fischer RS, Jensen RA
J Biol Chem 1992 Feb 5;267(4):2487-93
PubMed ID: 1733946
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994