Pseudomonas aeruginosa PAO1, PA3477 (rhlR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3477
Name
rhlR
Replicon chromosome
Genomic location 3889925 - 3890650 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252167.1
GI 15598673
Affymetrix PA3477_rhlR_at
Entrez 878968
GenBank AAG06865.1
INSDC AAA62130.1
NCBI Locus Tag PA3477
protein_id(GenBank) gb|AAG06865.1|AE004768_10|gnl|PseudoCAP|PA3477
TIGR NTL03PA03477
UniParc UPI000013387F
UniProtKB Acc P54292
UniProtKB ID RHLR_PSEAE
UniRef100 UniRef100_P54292
UniRef50 UniRef50_P54292
UniRef90 UniRef90_P54292

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator RhlR
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.59
Kyte-Doolittle Hydrophobicity Value -0.278
Molecular Weight (kDa) 27.6
Isoelectric Point (pI) 7.19

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8DQ1 TRANSCRIPTION/DNA 07/18/22 Quorum-sensing receptor RhlR bound to PqsE Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 4.1 ELECTRON MICROSCOPY 100.0
7R3J TRANSCRIPTION 02/07/22 Nativ complex of PqsE and RhlR with the synthetic antagonist mBTL Pseudomonas aeruginosa PAO1 3.06 X-RAY DIFFRACTION 100.0
7R3E TRANSCRIPTION 02/07/22 Fusion construct of PqsE and RhlR in complex with the synthetic antagonist mBTL Pseudomonas aeruginosa PAO1 3.46 X-RAY DIFFRACTION 100.0
8DQ0 TRANSCRIPTION 07/18/22 Quorum-sensing receptor RhlR bound to PqsE Pseudomonas aeruginosa 3.74 ELECTRON MICROSCOPY 100.0
8B4A SIGNALING PROTEIN 09/20/22 Nativ complex of PqsE and RhlR with autoinducer C4-HSL Pseudomonas aeruginosa PAO1 3.06 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0093
Category N-(butanoyl)-L-homoserine lactone QS system
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 188 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001096 (1439 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3477
Search term: rhlR

Human Homologs

References

Isolation and characterization of a regulatory gene affecting rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa.
Ochsner UA, Koch AK, Fiechter A, Reiser J
J Bacteriol 1994 Apr;176(7):2044-54
PubMed ID: 8144472