Pseudomonas aeruginosa PAO1, PA3527 (pyrC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3527
Name
pyrC
Replicon chromosome
Genomic location 3947095 - 3948141 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252217.1
GI 15598723
Affymetrix PA3527_pyrC_at
Entrez 879809
GenBank AAG06915.1
INSDC AAG06915.1
NCBI Locus Tag PA3527
protein_id(GenBank) gb|AAG06915.1|AE004773_4|gnl|PseudoCAP|PA3527
TIGR NTL03PA03527
UniParc UPI0000132C73
UniProtKB Acc P72170
UniProtKB ID PYRC_PSEAE
UniRef100 UniRef100_P72170
UniRef50 UniRef50_P06204
UniRef90 UniRef90_P72170

Product

Feature Type CDS
Coding Frame 1
Product Name
dihydroorotase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.75
Kyte-Doolittle Hydrophobicity Value -0.160
Molecular Weight (kDa) 38.4
Isoelectric Point (pI) 6.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 190 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002138 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3527
Search term: pyrC
Search term: dihydroorotase

Human Homologs

References

Nucleotide sequence of the structural gene for dihydroorotase of Escherichia coli K12.
Bäckström D, Sjöberg RM, Lundberg LG
Eur J Biochem 1986 Oct 1;160(1):77-82
PubMed ID: 2876892
Nucleotide sequence and expression of the pyrC gene of Escherichia coli K-12.
Wilson HR, Chan PT, Turnbough CL Jr
J Bacteriol 1987 Jul;169(7):3051-8
PubMed ID: 2885307